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IHCeasy JMY Ready-To-Use IHC Kit

JMY Ready-to-use reagent kit for IHC.

Reactivity

Human, Mouse, Rat

Sample Type

FFPE tissue

Cat No : KHC1267

Synonyms

JMY, WHDC1L3

Validation Data Gallery

  • Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using KHC1267 (JMY IHC Kit).

    Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using KHC1267 (JMY IHC Kit).

  • Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using KHC1267 (JMY IHC Kit).

    Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using KHC1267 (JMY IHC Kit).

  • Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using KHC1267 (JMY IHC Kit).

    Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using KHC1267 (JMY IHC Kit).

  • Immunohistochemical analysis of paraffin-embedded mouse colon tissue slide using KHC1267 (JMY IHC Kit).

    Immunohistochemical analysis of paraffin-embedded mouse colon tissue slide using KHC1267 (JMY IHC Kit).

  • Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using KHC1267 (JMY IHC Kit).

    Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using KHC1267 (JMY IHC Kit).

  • Immunohistochemical analysis of paraffin-embedded rat colon tissue slide using KHC1267 (JMY IHC Kit).

    Immunohistochemical analysis of paraffin-embedded rat colon tissue slide using KHC1267 (JMY IHC Kit).

  • Immunohistochemical analysis of paraffin-embedded rat testis tissue slide using KHC1267 (JMY IHC Kit).

    Immunohistochemical analysis of paraffin-embedded rat testis tissue slide using KHC1267 (JMY IHC Kit).

  • Components in IHCeasy ready-to-use IHC kit.

Product Information

KHC1267 is a ready-to-use IHC kit for staining of JMY. The kit provides all reagents, from antigen retrieval to cover slip mounting, that require little to no diluting or handling prior to use. Simply apply the reagents to your sample slide according to the protocol and you're steps away from obtaining high-quality IHC data.

Product name IHCeasy JMY Ready-To-Use IHC Kit
Sample type FFPE tissue
Assay type Immunohistochemistry
Primary antibody type Rabbit Polyclonal
Secondary antibody type Polymer-HRP-Goat anti-Rabbit
Reactivity Human, Mouse, Rat

Kit components

ComponentSizeConcentration
Antigen Retrieval Buffer100 mL50×
Washing Buffer100 mL ×220×
Blocking Buffer5 mLRTU
Primary Antibody5 mLRTU
Secondary Antibody5 mLRTU
Chromogen Component A0.2 mLRTU
Chromogen Component B4 mLRTU
Signal Enhancer5 mLRTU
Counter Staining Reagent5 mLRTU
Mounting Media5 mLRTU
Datasheet1 Copy
Manual1 Copy

Background Information

Junction mediating and regulatory protein(JMY) is a 988 amino acid protein, which contains one WH2 domain and belongs to the JMY family. IIn nucleus, JMY acts both a nuclear p53/TP53-cofactor and a cytoplasmic regulator of actin dynamics in response to cellular stress. In cytoplasm, JMY acts as a nucleation-promoting factor for both branched and unbranched actin filaments. Due to alternative splicing, several isoforms of JMY are produced, and these various isoforms have different influencing affects on p53 activation, with some isoforms markedly enhancing p53 responses compared to the other splicing variants.

Properties

Storage Instructions All the reagents are stored at 2-8°C. The kit is stable for 6 months from the date of receipt.
Synonyms JMY, WHDC1L3