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验证数据展示

  • IF Staining of Jurkat using CL488-24164

    IF Staining of Jurkat using CL488-24164

    Immunofluorescent analysis of (4% PFA) fixed Jurkat cells using CoraLite® Plus 488 JNK antibody (CL488-24164) at dilution of 1:200.

  • FC experiment of HeLa using CL488-24164

    FC experiment of HeLa using CL488-24164

    1X10^6 HeLa cells were intracellularly stained with 0.8 ug CoraLite® Plus 488 Anti-Human JNK (CL488-24164) (red), or 0.8 ug Isotype Control. Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

CoraLite® Plus 488-conjugated JNK Polyclonal antibody

JNK Polyclonal Antibody for IF/ICC, FC (Intra)
Cat No. CL488-24164

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

IF/ICC, FC (Intra)

别名

MAPK8, JNK1A2, JNK1, JNK 2, JNK 1

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  CoraLite® Plus 488
规格: 

-/ -

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经过测试的应用

Positive IF/ICC detected inJurkat cells
Positive FC (Intra) detected inHeLa cells
Planning an IHC experiment? We recommend our IHCeasy MAPK8/JNK Ready-To-Use IHC Kit. MAPK8/JNK primary antibody included.
For other applications, we recommend the unconjugated version of this antibody, 24164-1-AP

推荐稀释比

应用推荐稀释比
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.80 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL488-24164 targets JNK in IF/ICC, FC (Intra) applications and shows reactivity with human, mouse, rat samples.

经测试应用 IF/ICC, FC (Intra) Application Description
经测试反应性 human, mouse, rat
免疫原 JNK fusion protein Ag21426 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 mitogen-activated protein kinase 8
别名 MAPK8, JNK1A2, JNK1, JNK 2, JNK 1
计算分子量 48 kDa
观测分子量40 kDa, 48 kDa
GenBank蛋白编号BC130572
基因名称 JNK
Gene ID (NCBI) 5599
RRIDAB_3084079
偶联类型 CoraLite® Plus 488 Fluorescent Dye
最大激发/发射波长493 nm / 522 nm
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDP45983
储存缓冲液 PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA , pH 7.3
储存条件Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Function

JNK1, also known as mitogen-activated protein kinase 8 - MAPK8, is a serine/threonine-protein kinase. It is an important player in signaling transduction pathways governing cellular response to external stimuli, including ultraviolet radiation (UV), inflammatory cytokines, and reactive oxygen species (ROS). JNK1 phosphorylates several transcription factors, such as c-Jun, Elk-1, and ATF2, affecting their transcription activity.

Tissue specificity

JNK1 is ubiquitously expressed.

Involvement in disease

  • JNK-mediated phosphorylation governs a variety of important cellular processes, and abnormalities of JNK1 activity have been implicated in obesity and cancer.

Isoforms

There are four isoforms of JNK1 (PMID: 8654373), giving rise to 46 kDa and 55 kDa isoforms. JNK1 isoforms differ in their substrate specificity and tissue expression profiles.

Post-translational modifications

JNK1 is phosphorylated by MAPK kinases (MAPKK).

Cellular localization

JNK1 is present both in the nucleus and in the cytoplasm. Stress conditions and other stimuli can cause a shift in JNK1 subcellular distribution.  

实验方案

Product Specific Protocols
IF protocol for CL Plus 488 JNK antibody CL488-24164Download protocol
FC protocol for CL Plus 488 JNK antibody CL488-24164Download protocol
Standard Protocols
Click here to view our Standard Protocols