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JPH1 Polyclonal antibody

JPH1 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 25979-1-AP

Print datasheet

Synonyms

JP 1, JP1, JPH1, junctophilin 1, Junctophilin type 1

验证数据展示

  • WB analysis of HEK-293 using 25979-1-AP

    WB analysis of HEK-293 using 25979-1-AP

    HEK-293 cells were subjected to SDS PAGE followed by western blot with 25979-1-AP (JPH1 Antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • IHC staining of human skeletal muscle using 25979-1-AP

    IHC staining of human skeletal muscle using 25979-1-AP

    Immunohistochemical analysis of paraffin-embedded human skeletal muscle tissue slide using 25979-1-AP (JPH1 antibody) at dilution of 1:200 (under 10x lens).

  • IHC staining of human skeletal muscle using 25979-1-AP

    IHC staining of human skeletal muscle using 25979-1-AP

    Immunohistochemical analysis of paraffin-embedded human skeletal muscle tissue slide using 25979-1-AP (JPH1 antibody) at dilution of 1:200 (under 40x lens).

  • WB analysis using 25979-1-AP

    WB analysis using 25979-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 25979-1-AP (JPH1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

经过测试的应用

Positive WB detected inHEK-293 cells, mouse heart tissue, rat heart tissue
Positive IHC detected inhuman skeletal muscle tissue, human skin tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:4000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

25979-1-AP targets JPH1 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications WB, IHC, ELISA Application Description
Tested Reactivity human, mouse, rat
Immunogen JPH1 fusion protein Ag23270 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name junctophilin 1
Synonyms JP 1, JP1, JPH1, junctophilin 1, Junctophilin type 1
Calculated Molecular Weight 72 kDa
Observed Molecular Weight 72 kDa
GenBank Accession NumberBC140875
Gene Symbol JPH1
Gene ID (NCBI) 56704
RRIDAB_2880317
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ9HDC5
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Junctophilin-1 is a protein that in humans is encoded by the JPH1 gene. This gene is a member of the junctophilin gene family. Junctional complexes between the plasma membrane and endoplasmic/sarcoplasmic reticulum are a common feature of all excitable cell types and mediate cross talk between cell surface and intracellular ion channels. The protein encoded by this gene is a component of junctional complexes and is composed of a C-terminal hydrophobic segment spanning the endoplasmic/sarcoplasmic reticulum membrane and a remaining cytoplasmic domain that shows specific affinity for the plasma membrane.

实验方案

Product Specific Protocols
WB protocol for JPH1 antibody 25979-1-APDownload protocol
IHC protocol for JPH1 antibody 25979-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols