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验证数据展示

  • WB analysis using CL488-66313

    WB analysis using CL488-66313

    Various lysates were subjected to SDS PAGE followed by western blot with CL488-66313 (JUN antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • FC experiment of NIH/3T3 using CL488-66313

    FC experiment of NIH/3T3 using CL488-66313

    1X10^6 NIH/3T3 cells were intracellularly stained with 0.4 ug CoraLite® Plus 488 Anti-Human JUN (CL488-66313, Clone:1G6B11) (red), or 0.4 ug Control Antibody. Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

CoraLite® Plus 488-conjugated JUN Monoclonal antibody

JUN Monoclonal Antibody for WB, FC (Intra)
Cat No. CL488-66313

产品说明书

CloneNo. 1G6B11

宿主/亚型

Mouse / IgG1

种属反应性

human, mouse, rat

应用

WB, FC (Intra)

别名

AP1, AP 1, jun oncogene, c Jun, Activator protein 1

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  CoraLite® Plus 488
规格: 

-/ -

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经过测试的应用

Positive WB detected inRAW 264.7 cells
Positive FC (Intra) detected inNIH/3T3 cells
Planning an IHC experiment? We recommend our IHCeasy JUN Ready-To-Use IHC Kit. JUN primary antibody included.
For other applications, we recommend the unconjugated version of this antibody, 66313-1-Ig

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:1000
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL488-66313 targets JUN in WB, FC (Intra) applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, FC (Intra) Application Description
经测试反应性 human, mouse, rat
免疫原 JUN fusion protein Ag17639 种属同源性预测
宿主/亚型 Mouse / IgG1
抗体类别 Monoclonal
产品类型 Antibody
全称 jun oncogene
别名 AP1, AP 1, jun oncogene, c Jun, Activator protein 1
计算分子量 331 aa, 36 kDa
观测分子量 39 kDa
GenBank蛋白编号BC068522
基因名称 JUN
Gene ID (NCBI) 3725
RRIDAB_3672909
偶联类型 CoraLite® Plus 488 Fluorescent Dye
最大激发/发射波长493 nm / 522 nm
形式 Liquid
纯化方式Protein G purification
UNIPROT IDP05412
储存缓冲液 PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
储存条件Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

What is the molecular weight of JUN?

The molecular weight of JUN is approximately 39 kDa.

What is the cellular localization of JUN?

JUN expression can be located in the nucleus. However, ubiquitinated JUN colocalizes with lysosomal proteins (PMID: 15469925).

 What is the function of JUN?

JUN is a basic leucine zipper (bZIP) transcription factor that recognizes and binds to a consensus heptamer motif of 5'-TGA[CG]TCA-3' in the enhancer region of target genes. JUN acts as either a homo- or heterodimer and binds to the DNA to regulate transcriptional activity, specifically promoting the activity of NR5A1 when phosphorylated by HIPK3, leading to increased steroidogenic gene expression upon the stimulation of the cAMP signaling pathway (PMID: 9732876).

 What post-translational modifications is JUN subjected to?

JUN is acetylated at Lys-271 by EP300 (PMID: 11689449). JUN is phosphorylated by PLK3 following exposure to hypoxia or UV irradiation, which leads to increased DNA-binding activity (PMID: 27281822), and also by PAK2 at numerous threonine residues to promote cell proliferation and transformation (PMID: 21177766). JUN is also phosphorylated by DYRK2 at Ser-243, which primes it for subsequent further phosphorylation by GSK3B and reduces its ability to bind DNA (PMID: 22307329).

 What is the role of JUN in disease?

Activation of JUN is linked with proliferation and angiogenesis in invasive breast cancers (PMID: 16733206).


实验方案

Product Specific Protocols
WB protocol for CL Plus 488 JUN antibody CL488-66313Download protocol
FC protocol for CL Plus 488 JUN antibody CL488-66313Download protocol
Standard Protocols
Click here to view our Standard Protocols