验证数据展示
经过测试的应用
| Positive WB detected in | HEK-293 cells, HT-29 cells |
Mouse monoclonal antibodies of IgA isotype can be detected with "anti-mouse IgG (H+L)" secondary antibodies.
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:5000-1:50000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
66890-1-Ig targets Jagged1 in WB, ELISA applications and shows reactivity with Human samples.
| 经测试应用 | WB, ELISA Application Description |
| 文献引用应用 | WB |
| 经测试反应性 | Human |
| 文献引用反应性 | human, goat |
| 免疫原 | Jagged1 fusion protein Ag11891 种属同源性预测 |
| 宿主/亚型 | Mouse / IgA |
| 抗体类别 | Monoclonal |
| 产品类型 | Antibody |
| 全称 | jagged 1 (Alagille syndrome) |
| 别名 | AGS, AHD, AWS, CD339, HJ1, JAG1, jagged 1 (Alagille syndrome), Jagged1, JAGL1, Protein jagged 1 |
| 计算分子量 | 1218 aa, 134 kDa |
| 观测分子量 | 150 kDa |
| GenBank蛋白编号 | BC098393 |
| 基因名称 | Jagged1 |
| Gene ID (NCBI) | 182 |
| RRID | AB_2882220 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Thiophilic affinity chromatograph |
| UNIPROT ID | P78504 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
Jagged1 is a type I, membrane- anchored, multidomain protein and a ligand of the Notch receptor. The receptor-ligand interaction can trigger the Notch signaling and activate transcription factors that play key roles in cell differentiation and morphogenesis. The predicted MW of Jagged1 is 134 kDa, while higher MW around 150-180 kDa had been observed in literations (PMID: 9716576, 9596653).
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for Jagged1 antibody 66890-1-Ig | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Mol Oncol Ryanodine receptor 2 promotes colorectal cancer metastasis by the ROS/BACH1 axis | ||
Int J Biol Sci Linc-KILH potentiates Notch1 signaling through inhibiting KRT19 phosphorylation and promotes the malignancy of hepatocellular carcinoma. | ||
Commun Biol Generation and characterization of cardiac valve endothelial-like cells from human pluripotent stem cells. | ||
Front Med (Lausanne) IL-7-Treated Periodontal Ligament Cells Regulate Local Immune Homeostasis by Modulating Treg/Th17 Cell Polarization. | ||
Cell Biochem Funct LncRNA TDRG1/miR-214-5p axis affects preeclampsia by modulating trophoblast cells. | ||
Acta Histochem miR-214-5p suppresses the proliferation, migration and invasion of trophoblast cells in pre-eclampsia by targeting jagged 1 to inhibit notch signaling pathway. |
