• All
  • Primary Antibodies
  • Conjugated Antibodies for IF
  • Conjugated Antibodies for FC
  • Secondary Antibodies
  • Antibody Labeling KitsNew
  • ELISA Kits
  • IHC Kits
  • Magnetic Cell Separation Kits
  • Cytokines & Growth Factors
  • Neutralizing/activating Antibodies
  • Nanobody-based Reagents
  • Accessory Products and Kits
  • Fusion Proteins
×
×
Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0

Jagged1 Recombinant antibody

Jagged1 Recombinant Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, ELISA

Conjugate

Unconjugated

CloneNo.

241412F1

Cat no : 84694-1-RR

Print datasheet

Synonyms

JAG1, Protein jagged-1, HJ1, CD339, 241412F1

验证数据展示

  • WB analysis of HepG2 using 84694-1-RR

    WB analysis of HepG2 using 84694-1-RR

    HepG2 cells were subjected to SDS PAGE followed by western blot with 84694-1-RR (Jagged1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Affinity and Kinetic Characterization of 84694-1-RR

    Affinity and Kinetic Characterization of 84694-1-RR

    Biolayer interferometry (BLl) kinetic assays of 84694-1-RR against Human Jagged1 were performed. The affinity constant is 0.688 nM.

经过测试的应用

Positive WB detected inHepG2 cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

84694-1-RR targets Jagged1 in WB, ELISA applications and shows reactivity with human samples.

Tested Applications WB, ELISA Application Description
Tested Reactivity human
Immunogen Recombinant Protein 种属同源性预测
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Full Name jagged 1 (Alagille syndrome)
Synonyms JAG1, Protein jagged-1, HJ1, CD339, 241412F1
Calculated Molecular Weight 1218 aa, 134 kDa
Observed Molecular Weight150 kDa
GenBank Accession NumberBC098393
Gene Symbol Jagged1
Gene ID (NCBI) 182
RRIDAB_3672110
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
UNIPROT IDP78504
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Jagged1 is a type I, membrane- anchored, multidomain protein and a ligand of the Notch receptor. The receptor-ligand interaction can trigger the Notch signaling and activate transcription factors that play key roles in cell differentiation and morphogenesis. The predicted MW of Jagged1 is 134 kDa, while higher MW around 150-180 kDa had been observed in literations (PMID: 9716576, 9596653).

实验方案

Product Specific Protocols
WB protocol for Jagged1 antibody 84694-1-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols