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验证数据展示

  • WB analysis of HepG2 using 84694-1-RR

    WB analysis of HepG2 using 84694-1-RR

    HepG2 cells were subjected to SDS PAGE followed by western blot with 84694-1-RR (Jagged1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Affinity and Kinetic Characterization of 84694-1-RR

    Affinity and Kinetic Characterization of 84694-1-RR

    Biolayer interferometry (BLl) kinetic assays of 84694-1-RR against Human Jagged1 were performed. The affinity constant is 0.688 nM.

Jagged1 Recombinant antibody

Jagged1 Recombinant Antibody for WB, ELISA
Cat No. 84694-1-RR

产品说明书

CloneNo. 241412F1

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, ELISA

别名

JAG1, Protein jagged-1, HJ1, CD339, 241412F1

缓冲液配方:  PBS and Azide
PBS and Azide
PBS Only
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inHepG2 cells

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:2000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

84694-1-RR targets Jagged1 in WB, ELISA applications and shows reactivity with human samples.

经测试应用 WB, ELISA Application Description
经测试反应性 human
免疫原 Recombinant Protein 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 jagged 1 (Alagille syndrome)
别名 JAG1, Protein jagged-1, HJ1, CD339, 241412F1
计算分子量 1218 aa, 134 kDa
观测分子量150 kDa
GenBank蛋白编号BC098393
基因名称 Jagged1
Gene ID (NCBI) 182
RRIDAB_3672110
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purfication
UNIPROT IDP78504
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Jagged1 is a type I, membrane- anchored, multidomain protein and a ligand of the Notch receptor. The receptor-ligand interaction can trigger the Notch signaling and activate transcription factors that play key roles in cell differentiation and morphogenesis. The predicted MW of Jagged1 is 134 kDa, while higher MW around 150-180 kDa had been observed in literations (PMID: 9716576, 9596653).

实验方案

Product Specific Protocols
WB protocol for Jagged1 antibody 84694-1-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols