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验证数据展示

  • FC experiment of HeLa using CL555-66630

    FC experiment of HeLa using CL555-66630

    1X10^6 HeLa cells were intracellularly stained with 0.4 ug CoraLite®555 Anti-Human KAP1 (CL555-66630, Clone:1B9G12) (red), or 0.4 ug Control Antibody. Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

  • IF Staining of HepG2 using CL555-66630

    IF Staining of HepG2 using CL555-66630

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using CoraLite®555 KAP1 antibody (CL555-66630, Clone: 1B9G12 ) at dilution of 1:200, CL488-phalloidin (green).

  • Featured Product
  • KD/KO验证

CoraLite®555-conjugated KAP1 Monoclonal antibody

KAP1 Monoclonal Antibody for IF/ICC, FC (Intra)
Cat No. CL555-66630

产品说明书

CloneNo. 1B9G12

宿主/亚型

Mouse / IgG2a

种属反应性

human

应用

IF/ICC, FC (Intra)

别名

TRIM28, KRAB associated protein 1, KAP-1, KAP 1, EC:2.3.2.27

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  CoraLite®555
规格: 

-/ -

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经过测试的应用

Positive IF/ICC detected inHepG2 cells
Positive FC (Intra) detected inHeLa cells
Planning an IHC experiment? We recommend our IHCeasy KAP1 Ready-To-Use IHC Kit. KAP1 primary antibody included.
For other applications, we recommend the unconjugated version of this antibody, 66630-1-Ig

推荐稀释比

应用推荐稀释比
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL555-66630 targets KAP1 in IF/ICC, FC (Intra) applications and shows reactivity with human samples.

经测试应用 IF/ICC, FC (Intra) Application Description
经测试反应性 human
免疫原 KAP1 fusion protein Ag7519 种属同源性预测
宿主/亚型 Mouse / IgG2a
抗体类别 Monoclonal
产品类型 Antibody
全称 tripartite motif-containing 28
别名 TRIM28, KRAB associated protein 1, KAP-1, KAP 1, EC:2.3.2.27
计算分子量 89 kDa
观测分子量 100 kDa
GenBank蛋白编号BC004978
基因名称 KAP1
Gene ID (NCBI) 10155
RRIDAB_2919691
偶联类型 CoraLite®555 Fluorescent Dye
最大激发/发射波长557 nm / 570 nm
形式 Liquid
纯化方式Protein A purification
UNIPROT IDQ13263
储存缓冲液 PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA , pH 7.3
储存条件Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

KAP1, also named as TRIM28 or RNF96, is a 835 amino acid protein, which contain one RING-type zinc finger, one PHD-type zinc finger, one bromo domain and two B box-type zinc fingers. KAP1 localizes in the nucleus and Belongs to the TRIM/RBCC family. KAP1 is a nuclear corepressor for KRAB domain-containing zinc finger proteins and mediates gene silencing by recruiting CHD3, a subunit of the nucleosome remodeling and deacetylation (NuRD) complex, and SETDB1 to the promoter regions of KRAB target genes. KAP1 is expressed in all tissues tested including spleen, thymus, prostate, testis, ovary, small intestine, colon and peripheral blood leukocytes. The calcualted molecular weight of KAP1 is 89 kDa, but modified KAP1 is about 100 kDa. (PMID: 18590578 )

实验方案

Product Specific Protocols
IF protocol for CL555 KAP1 antibody CL555-66630Download protocol
FC protocol for CL555 KAP1 antibody CL555-66630Download protocol
Standard Protocols
Click here to view our Standard Protocols