验证数据展示
经过测试的应用
| Positive WB detected in | HeLa cells, MCF-7 cells, HSC-T6 cells, NIH/3T3 cells, SKOV-3 cells |
| Positive IF/ICC detected in | SKOV-3 cells |
This antibody may not be suitable for IHC test.
Planning an IF experiment? We recommend our CoraLite® Plus 488 and CoraLite® Plus 750 conjugated versions of this antibody.
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:2000-1:10000 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:200-1:800 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
66575-1-Ig targets KAT2A/GCN5 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with Human, mouse, rat samples.
| 经测试应用 | WB, IF/ICC, ELISA Application Description |
| 文献引用应用 | WB, IHC, IF, IP |
| 经测试反应性 | Human, mouse, rat |
| 文献引用反应性 | human, mouse, rat |
| 免疫原 | KAT2A/GCN5 fusion protein Ag6940 种属同源性预测 |
| 宿主/亚型 | Mouse / IgG2a |
| 抗体类别 | Monoclonal |
| 产品类型 | Antibody |
| 全称 | K(lysine) acetyltransferase 2A |
| 别名 | GCN5, GCN5L2, hGCN5, Histone acetyltransferase GCN5, HsGCN5, K(lysine) acetyltransferase 2A, KAT2A, KAT2A/GCN5, Lysine acetyltransferase 2A, PCAF b, STAF97 |
| 计算分子量 | 94 kDa |
| 观测分子量 | 94 kDa |
| GenBank蛋白编号 | BC032743 |
| 基因名称 | KAT2A |
| Gene ID (NCBI) | 2648 |
| RRID | AB_2881935 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Protein A purification |
| UNIPROT ID | Q92830 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
GCN5, encoded by KAT2A, is a member of nuclear A-type histone acetyltransferase (HAT) which has direct impact on gene transcription. Additionally, GCN5 has roles in cell cycle regulation, DNA replication, and DNA repair, highlighting it as key player in the maintenance of genome stability. GCN5 is a coactivator of c-MYC oncogene protein and has oncogenic roles in various cancers.
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for KAT2A/GCN5 antibody 66575-1-Ig | Download protocol |
| IF protocol for KAT2A/GCN5 antibody 66575-1-Ig | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Brain Behav Immun Kynurenine pathway is altered in BDNF Val66Met knock-in mice: Effect of physical exercise. | ||
J Bone Miner Res Inhibition of ACLY Leads to Suppression of Osteoclast Differentiation and Function Via Regulation of Histone Acetylation. | ||
Development Dynamic profiling and functional interpretation of histone Kcr and Kla during neural development.
| ||
PLoS One Psychostimulants and opioids differentially influence the epigenetic modification of histone acetyltransferase and histone deacetylase in astrocytes. | ||
BMC Cancer Histone regulator KAT2A acts as a potential biomarker related to tumor microenvironment and prognosis of diffuse large B cell lymphoma
| ||
Sci Rep SF3B4 downregulation restrains lung adenocarcinoma tumorigenesis via 5' alternative splicing of KAT2A |
