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  • Featured Product
  • KD/KO Validated

CoraLite® Plus 750-conjugated KAT2A/GCN5 Monoclonal antibody

KAT2A/GCN5 Monoclonal Antibody for WB

Host / Isotype

Mouse / IgG2a

Reactivity

Human, mouse, rat

Applications

WB

Conjugate

CoraLite® Plus 750 Fluorescent Dye

CloneNo.

1F6G7

Cat no : CL750-66575

Print datasheet

Synonyms

GCN5, GCN5L2, hGCN5, Histone acetyltransferase GCN5, HsGCN5, K(lysine) acetyltransferase 2A, KAT2A, Lysine acetyltransferase 2A, PCAF b, STAF97

验证数据展示

  • WB analysis using CL750-66575

    WB analysis using CL750-66575

    Various lysates were subjected to SDS PAGE followed by western blot with CL750-66575 (KAT2A/GCN5 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

经过测试的应用

Positive WB detected inHeLa cells, NIH/3T3 cells

This antibody may not be suitable for IHC test.

For other applications, we recommend the unconjugated version of this antibody, 66575-1-Ig

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL750-66575 targets KAT2A/GCN5 in WB applications and shows reactivity with Human, mouse, rat samples.

Tested Applications WB Application Description
Tested Reactivity Human, mouse, rat
Immunogen KAT2A/GCN5 fusion protein Ag6940 种属同源性预测
Host / Isotype Mouse / IgG2a
Class Monoclonal
Type Antibody
Full Name K(lysine) acetyltransferase 2A
Synonyms GCN5, GCN5L2, hGCN5, Histone acetyltransferase GCN5, HsGCN5, K(lysine) acetyltransferase 2A, KAT2A, Lysine acetyltransferase 2A, PCAF b, STAF97
Calculated Molecular Weight 94 kDa
Observed Molecular Weight 94 kDa
GenBank Accession NumberBC032743
Gene Symbol KAT2A
Gene ID (NCBI) 2648
Conjugate CoraLite® Plus 750 Fluorescent Dye
Excitation/Emission Maxima Wavelengths755 nm / 780 nm
Form Liquid
Purification MethodProtein A purification
UNIPROT IDQ92830
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

GCN5, encoded by KAT2A, is a member of nuclear A-type histone acetyltransferase (HAT) which has direct impact on gene transcription. Additionally, GCN5 has roles in cell cycle regulation, DNA replication, and DNA repair, highlighting it as key player in the maintenance of genome stability. GCN5 is a coactivator of c-MYC oncogene protein and has oncogenic roles in various cancers.

实验方案

Product Specific Protocols
WB protocol for CL Plus 750 KAT2A/GCN5 antibody CL750-66575Download protocol
Standard Protocols
Click here to view our Standard Protocols