验证数据展示
经过测试的应用
| Positive WB detected in | HeLa cells, NIH/3T3 cells |
This antibody may not be suitable for IHC test.
For other applications, we recommend the unconjugated version of this antibody, 66575-1-Ig
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:500-1:1000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
CL750-66575 targets KAT2A/GCN5 in WB applications and shows reactivity with Human, mouse, rat samples.
| 经测试应用 | WB Application Description |
| 经测试反应性 | Human, mouse, rat |
| 免疫原 | KAT2A/GCN5 fusion protein Ag6940 种属同源性预测 |
| 宿主/亚型 | Mouse / IgG2a |
| 抗体类别 | Monoclonal |
| 产品类型 | Antibody |
| 全称 | K(lysine) acetyltransferase 2A |
| 别名 | GCN5, GCN5L2, hGCN5, Histone acetyltransferase GCN5, HsGCN5, K(lysine) acetyltransferase 2A, KAT2A, Lysine acetyltransferase 2A, PCAF b, STAF97 |
| 计算分子量 | 94 kDa |
| 观测分子量 | 94 kDa |
| GenBank蛋白编号 | BC032743 |
| 基因名称 | KAT2A |
| Gene ID (NCBI) | 2648 |
| RRID | AB_3673718 |
| 偶联类型 | CoraLite® Plus 750 Fluorescent Dye |
| 最大激发/发射波长 | 755 nm / 780 nm |
| 形式 | Liquid |
| 纯化方式 | Protein A purification |
| UNIPROT ID | Q92830 |
| 储存缓冲液 | PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3. |
| 储存条件 | Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
GCN5, encoded by KAT2A, is a member of nuclear A-type histone acetyltransferase (HAT) which has direct impact on gene transcription. Additionally, GCN5 has roles in cell cycle regulation, DNA replication, and DNA repair, highlighting it as key player in the maintenance of genome stability. GCN5 is a coactivator of c-MYC oncogene protein and has oncogenic roles in various cancers.
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for CL Plus 750 KAT2A/GCN5 antibody CL750-66575 | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |