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验证数据展示

  • WB analysis of HUVEC using 28390-1-AP (same clone as 28390-1-PBS)

    WB analysis of HUVEC using 28390-1-AP (same clone as 28390-1-PBS)

    WB result of KAT2A/GCN5 antibody (28390-1-AP; 1:4000; room temperature for 1.5 hours) with wild-type and KAT2A/GCN5 knockout HUVEC cells. This data was developed using the same antibody clone with 28390-1-PBS in a different storage buffer formulation.

  • WB analysis using 28390-1-AP (same clone as 28390-1-PBS)

    WB analysis using 28390-1-AP (same clone as 28390-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 28390-1-AP (KAT2A/GCN5 antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 28390-1-PBS in a different storage buffer formulation.

  • IF Staining of HeLa using 28390-1-AP (same clone as 28390-1-PBS)

    IF Staining of HeLa using 28390-1-AP (same clone as 28390-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using KAT2A/GCN5 antibody (28390-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red). This data was developed using the same antibody clone with 28390-1-PBS in a different storage buffer formulation.

  • IP experiment of LNCaP using 28390-1-AP (same clone as 28390-1-PBS)

    IP experiment of LNCaP using 28390-1-AP (same clone as 28390-1-PBS)

    IP result of anti-KAT2A/GCN5 (IP:28390-1-AP, 4ug; Detection:28390-1-AP 1:6000) with LNCaP cells lysate 1200 ug. This data was developed using the same antibody clone with 28390-1-PBS in a different storage buffer formulation.

  • Featured Product
  • KD/KO验证

KAT2A/GCN5 Polyclonal antibody, PBS Only

KAT2A/GCN5 Polyclonal Antibody for WB, IF/ICC, IP, Indirect ELISA
Cat No. 28390-1-PBS

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, IF/ICC, IP, Indirect ELISA

别名

hGCN5, General control of amino acid synthesis protein 5-like 2, GCN5L2, GCN5, EC:2.3.1.48

缓冲液配方:  PBS Only
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格: 

-/ -

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产品信息

28390-1-PBS targets KAT2A/GCN5 in WB, IF/ICC, IP, Indirect ELISA applications and shows reactivity with human samples.

经测试应用 WB, IF/ICC, IP, Indirect ELISA Application Description
经测试反应性 human
免疫原 KAT2A/GCN5 fusion protein Ag29005 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 K(lysine) acetyltransferase 2A
别名 hGCN5, General control of amino acid synthesis protein 5-like 2, GCN5L2, GCN5, EC:2.3.1.48
计算分子量 94 kDa
观测分子量94 kDa
GenBank蛋白编号BC032743
基因名称 KAT2A
Gene ID (NCBI) 2648
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ92830
储存缓冲液 PBS only , pH 7.3
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

GCN5, encoded by KAT2A, is a member of nuclear A-type histone acetyltransferase (HAT) which has direct impact on gene transcription. Additionally, GCN5 has roles in cell cycle regulation, DNA replication, and DNA repair, highlighting it as key player in the maintenance of genome stability. GCN5 is a coactivator of c-MYC oncogene protein and has oncogenic roles in various cancers.