验证数据展示
经过测试的应用
| Positive IF/ICC detected in | HepG2 cells |
| Positive FC (Intra) detected in | HCT 116 cells |
推荐稀释比
| Application | Dilution |
|---|---|
| Immunofluorescence (IF)/ICC | IF/ICC : 1:200-1:800 |
| Flow Cytometry (FC) (INTRA) | FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
82937-1-RR targets TIP60/KAT5 in IF/ICC, FC (Intra), ELISA applications and shows reactivity with human samples.
| Tested Applications | IF/ICC, FC (Intra), ELISA Application Description |
| Tested Reactivity | human |
| Immunogen | TIP60/KAT5 fusion protein Ag1261 种属同源性预测 |
| Host / Isotype | Rabbit / IgG |
| Class | Recombinant |
| Type | Antibody |
| Full Name | K(lysine) acetyltransferase 5 |
| Synonyms | KAT5, EC:2.3.1.48, EC:2.3.1.-, cPLA2, cPLA(2)-interacting protein |
| Calculated Molecular Weight | 60 kDa |
| GenBank Accession Number | BC000166 |
| Gene Symbol | KAT5 |
| Gene ID (NCBI) | 10524 |
| RRID | AB_3670680 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Protein A purification |
| UNIPROT ID | Q92993 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
KAT5, also named as The histone acetyltransferase (HAT) Tat interactive protein 60 kD belongs to the MYST protein family that contains atypical zinc finger and histone acetyltransferase domains. Also, KAT5 contains a chromodomain. KAT5 is a catalytic subunit of the NuA4 histone acetyltransferase complex. The NuA4 HAT complex plays a role in transcriptional activation of select genes mainly by acetylation of nucleosomal histone H4 and H2A, which influence nucleosome-DNA interaction and promotes interaction of the modified histones with other proteins that could regulate transcription positively. This complex also involves in the activation of transcriptional programs associated with oncogene and proto-oncogene mediated growth induction, tumor suppressor mediated growth arrest and replicative senescence, DNA repair and apoptosis.
实验方案
| Product Specific Protocols | |
|---|---|
| IF protocol for TIP60/KAT5 antibody 82937-1-RR | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |