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  • Featured Product
  • KD/KO Validated

KBTBD2 Monoclonal antibody, PBS Only

KBTBD2 Monoclonal Antibody for WB, IF/ICC, Indirect ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human, mouse, rat

Applications

WB, IF/ICC, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

2A9F3

Cat no : 67895-1-PBS

Print datasheet

Synonyms

BKLHD1, KBTBD2, KIAA1489

验证数据展示

  • WB analysis using 67895-1-Ig (same clone as 67895-1-PBS)

    WB analysis using 67895-1-Ig (same clone as 67895-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 67895-1-Ig (KBTBD2 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated GAPDH Monoclonal antibody (HRP-60004) as loading control. This data was developed using the same antibody clone with 67895-1-PBS in a different storage buffer formulation.

  • IF Staining of HeLa using 67895-1-Ig (same clone as 67895-1-PBS)

    IF Staining of HeLa using 67895-1-Ig (same clone as 67895-1-PBS)

    Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using KBTBD2 antibody (67895-1-Ig, Clone: 2A9F3 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L), CL594-Phalloidin (red). DAPI (blue). This data was developed using the same antibody clone with 67895-1-PBS in a different storage buffer formulation.

产品信息

67895-1-PBS targets KBTBD2 in WB, IF/ICC, Indirect ELISA applications and shows reactivity with Human, mouse, rat samples.

Tested Applications WB, IF/ICC, Indirect ELISA Application Description
Tested Reactivity Human, mouse, rat
Immunogen KBTBD2 fusion protein Ag4933 种属同源性预测
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Full Name kelch repeat and BTB (POZ) domain containing 2
Synonyms BKLHD1, KBTBD2, KIAA1489
Calculated Molecular Weight 622 aa, 71 kDa
Observed Molecular Weight60-70 kDa
GenBank Accession NumberBC047107
Gene Symbol KBTBD2
Gene ID (NCBI) 25948
RRIDAB_2918651
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
UNIPROT IDQ8IY47
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

Kelch repeat and BTB domain containing 2(KBTBD2), also known as BTB and kelch domain-containing protein 1, encodes a BTB-Kelch family subunit that p85α-specific recognition for the Cullin3-based E3 ubiqitin ligase complex, which regulates p85α protein ubiquitination and subsequently regulates phosphoinositide-3 kinases (PI3K) signaling (PMID:27708159). However, the expression profile and functional significance of Kbtbd2 is still largely unknown. The Human Protein Atlas which is an online source shows high Kbtbd2 expression in the spleen and thymus, suggesting that Kbtbd2 might involved in immunization (PMID:29331106). In addition, Kbtbd2 is related to Src-induced phosphorylation of protein, spermatogonial stem cell activity(PMID:15623525, 29331106).