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KBTBD2 Monoclonal antibody
KBTBD2 Monoclonal Antibody for WB, IF/ICC, ELISA
Host / Isotype
Mouse / IgG1
Reactivity
Human, mouse, rat and More (1)
Applications
WB, IF/ICC, ELISA and More (1)
Conjugate
Unconjugated
CloneNo.
2A9F3
验证数据展示
经过测试的应用
| Positive WB detected in | A549 cells, U2OS cells, HeLa cells, K-562 cells, Jurkat cells, HSC-T6 cells |
| Positive IF/ICC detected in | HeLa cells |
Planning an IF experiment? We recommend our CoraLite® Plus 488 and CoraLite®594 conjugated versions of this antibody.
推荐稀释比
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:6000 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:200-1:800 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
发表文章中的应用
| KD/KO | See 1 publications below |
| WB | See 1 publications below |
| IHC | See 1 publications below |
产品信息
67895-1-Ig targets KBTBD2 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with Human, mouse, rat samples.
| Tested Applications | WB, IF/ICC, ELISA Application Description |
| Cited Applications | WB, IHC |
| Tested Reactivity | Human, mouse, rat |
| Cited Reactivity | human, mouse |
| Immunogen | KBTBD2 fusion protein Ag4933 种属同源性预测 |
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Full Name | kelch repeat and BTB (POZ) domain containing 2 |
| Synonyms | BKLHD1, KBTBD2, KIAA1489 |
| Calculated Molecular Weight | 622 aa, 71 kDa |
| Observed Molecular Weight | 60-70 kDa |
| GenBank Accession Number | BC047107 |
| Gene Symbol | KBTBD2 |
| Gene ID (NCBI) | 25948 |
| RRID | AB_2918651 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Protein G purification |
| UNIPROT ID | Q8IY47 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
Kelch repeat and BTB domain containing 2(KBTBD2), also known as BTB and kelch domain-containing protein 1, encodes a BTB-Kelch family subunit that p85α-specific recognition for the Cullin3-based E3 ubiqitin ligase complex, which regulates p85α protein ubiquitination and subsequently regulates phosphoinositide-3 kinases (PI3K) signaling (PMID:27708159). However, the expression profile and functional significance of Kbtbd2 is still largely unknown. The Human Protein Atlas which is an online source shows high Kbtbd2 expression in the spleen and thymus, suggesting that Kbtbd2 might involved in immunization (PMID:29331106). In addition, Kbtbd2 is related to Src-induced phosphorylation of protein, spermatogonial stem cell activity(PMID:15623525, 29331106).
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for KBTBD2 antibody 67895-1-Ig | Download protocol |
| IF protocol for KBTBD2 antibody 67895-1-Ig | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
