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验证数据展示

  • IF Staining of HeLa using CL594-67895

    IF Staining of HeLa using CL594-67895

    Immunofluorescent analysis of (-20°C Methanol) fixed HeLa cells using CoraLite®594 KBTBD2 antibody (CL594-67895, Clone: 2A9F3 ) at dilution of 1:200.

  • Featured Product
  • KD/KO验证

CoraLite®594-conjugated KBTBD2 Monoclonal antibody

KBTBD2 Monoclonal Antibody for IF/ICC
Cat No. CL594-67895

产品说明书

CloneNo. 2A9F3

宿主/亚型

Mouse / IgG1

种属反应性

Human, mouse, rat

应用

IF/ICC

别名

BKLHD1, KBTBD2, KIAA1489

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  CoraLite®594
规格: 

-/ -

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经过测试的应用

Positive IF/ICC detected inHeLa cells
For other applications, we recommend the unconjugated version of this antibody, 67895-1-Ig

推荐稀释比

应用推荐稀释比
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL594-67895 targets KBTBD2 in IF/ICC applications and shows reactivity with Human, mouse, rat samples.

经测试应用 IF/ICC Application Description
经测试反应性 Human, mouse, rat
免疫原 KBTBD2 fusion protein Ag4933 种属同源性预测
宿主/亚型 Mouse / IgG1
抗体类别 Monoclonal
产品类型 Antibody
全称 kelch repeat and BTB (POZ) domain containing 2
别名 BKLHD1, KBTBD2, KIAA1489
计算分子量 622 aa, 71 kDa
观测分子量 71 kDa
GenBank蛋白编号BC047107
基因名称 KBTBD2
Gene ID (NCBI) 25948
RRIDAB_2923931
偶联类型 CoraLite®594 Fluorescent Dye
最大激发/发射波长588 nm / 604 nm
形式 Liquid
纯化方式Protein G purification
UNIPROT IDQ8IY47
储存缓冲液 PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA , pH 7.3
储存条件Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Kelch repeat and BTB domain containing 2(KBTBD2), also known as BTB and kelch domain-containing protein 1, encodes a BTB-Kelch family subunit that p85α-specific recognition for the Cullin3-based E3 ubiqitin ligase complex, which regulates p85α protein ubiquitination and subsequently regulates phosphoinositide-3 kinases (PI3K) signaling (PMID:27708159). However, the expression profile and functional significance of Kbtbd2 is still largely unknown. The Human Protein Atlas which is an online source shows high Kbtbd2 expression in the spleen and thymus, suggesting that Kbtbd2 might involved in immunization (PMID:29331106). In addition, Kbtbd2 is related to Src-induced phosphorylation of protein, spermatogonial stem cell activity(PMID:15623525, 29331106).

实验方案

Product Specific Protocols
IF protocol for CL594 KBTBD2 antibody CL594-67895Download protocol
Standard Protocols
Click here to view our Standard Protocols