验证数据展示
经过测试的应用
Positive WB detected in | Daudi cells, RKO cells, U-118 MG cells |
推荐稀释比
应用 | 推荐稀释比 |
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Western Blot (WB) | WB : 1:500-1:3000 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
产品信息
31970-1-AP targets KBTBD8 in WB, ELISA applications and shows reactivity with human samples.
经测试应用 | WB, ELISA Application Description |
经测试反应性 | human |
免疫原 | KBTBD8 fusion protein Ag36219 种属同源性预测 |
宿主/亚型 | Rabbit / IgG |
抗体类别 | Polyclonal |
产品类型 | Antibody |
全称 | kelch repeat and BTB (POZ) domain containing 8 |
别名 | T-cell activation kelch repeat protein, TA-KRP, TAKRP, KIAA1842, Kelch repeat and BTB domain-containing protein 8 |
计算分子量 | 69 kDa,601aa |
观测分子量 | 60 kDa |
GenBank蛋白编号 | NM_032505.2 |
基因名称 | KBTBD8 |
Gene ID (NCBI) | 84541 |
RRID | AB_3670161 |
偶联类型 | Unconjugated |
形式 | Liquid |
纯化方式 | Antigen affinity Purification |
UNIPROT ID | Q8NFY9 |
储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
Kelch repeat and BTB domain containing 8 (KBTBD8, also known as TAKRP and TA-KRP) is a member of the BTB-kelch family of proteins, which is specifically localized at the Golgi apparatus in nondividing cells and becomes restricted to the spindle apparatus upon mitosis (PMID: 23578279). KBTBD family proteins are involved in ubiquitination in various cells and tissues and are correlated with human muscle diseases (PMID: 9878064; 16547521; 24959344). The expression level of KBTBD8 might affect the proliferation and migration of epithelial ovarian cancer (PMID: 33109073).
实验方案
Product Specific Protocols | |
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WB protocol for KBTBD8 antibody 31970-1-AP | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |