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Kir2.2 Polyclonal antibody

Kir2.2 Polyclonal Antibody for WB, FC (Intra), ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, FC (Intra), ELISA

Conjugate

Unconjugated

Cat no : 14135-1-AP

Print datasheet

Synonyms

KCNJ12, IRK2, IRK 2, hkir2.2x, hIRK1

验证数据展示

  • WB analysis of mouse lung using 14135-1-AP

    WB analysis of mouse lung using 14135-1-AP

    mouse lung tissue were subjected to SDS PAGE followed by western blot with 14135-1-AP (Kir2.2 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

  • FC experiment of HEK-293 using 14135-1-AP

    FC experiment of HEK-293 using 14135-1-AP

    1x10^6 HEK-293 cells were intracellularly stained with 0.5 ug Kir2.2 Polyclonal antibody (14135-1-AP) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.5 ug Rabbit IgG control Rabbit PolyAb (30000-0-AP) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

经过测试的应用

Positive WB detected inmouse lung tissue, rat lung tissue
Positive FC (Intra) detected inHEK-293 cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2400
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.50 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

14135-1-AP targets Kir2.2 in WB, FC (Intra), ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications WB, FC (Intra), ELISA Application Description
Tested Reactivity human, mouse, rat
Immunogen Kir2.2 fusion protein Ag5349 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name potassium inwardly-rectifying channel, subfamily J, member 12
Synonyms KCNJ12, IRK2, IRK 2, hkir2.2x, hIRK1
Calculated Molecular Weight 433 aa, 49 kDa
Observed Molecular Weight 45-50 kDa
GenBank Accession NumberBC027982
Gene Symbol Kir2.2
Gene ID (NCBI) 3768
RRIDAB_2130478
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ14500
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for Kir2.2 antibody 14135-1-APDownload protocol
FC protocol for Kir2.2 antibody 14135-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols