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Kir7.1 Polyclonal antibody

Kir7.1 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 12657-1-AP

Print datasheet

Synonyms

KCNJ13, KIR1.4, Kir7.1, SVD

验证数据展示

  • WB analysis of human brain using 12657-1-AP

    WB analysis of human brain using 12657-1-AP

    human brain tissue were subjected to SDS PAGE followed by western blot with 12657-1-AP (Kir7.1 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • IHC staining of human small intestine using 12657-1-AP

    IHC staining of human small intestine using 12657-1-AP

    Immunohistochemical analysis of paraffin-embedded human small intestine tissue slide using 12657-1-AP (Kir7.1 antibody at dilution of 1:50 (under 10x lens).

  • IHC staining of human small intestine using 12657-1-AP

    IHC staining of human small intestine using 12657-1-AP

    Immunohistochemical analysis of paraffin-embedded human small intestine tissue slide using 12657-1-AP (Kir7.1 antibody at dilution of 1:50 (under 40x lens).

经过测试的应用

Positive WB detected inhuman brain tissue, human kidney tissue
Positive IHC detected inhuman small intestine tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:200-1:1000
Immunohistochemistry (IHC)IHC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

IHCSee 1 publications below

产品信息

12657-1-AP targets Kir7.1 in WB, IHC, ELISA applications and shows reactivity with human samples.

Tested Applications WB, IHC, ELISA Application Description
Cited ApplicationsIHC
Tested Reactivity human
Cited Reactivityhuman
Immunogen Kir7.1 fusion protein Ag3327 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name potassium inwardly-rectifying channel, subfamily J, member 13
Synonyms KCNJ13, KIR1.4, Kir7.1, SVD
Calculated Molecular Weight 360 aa, 43 kDa
Observed Molecular Weight 43 kDa
GenBank Accession NumberBC037290
Gene Symbol Kir7.1
Gene ID (NCBI) 3769
RRIDAB_2130488
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDO60928
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Kir7.1, encoded by KCNJ13 gene, is a member of the inwardly rectifying potassium channel family of proteins. Kir7.1 is a low-conductance channel. At mRNA level, Kir7.1 is predominantly expressed in small intestine and also expressed in stomach, kidney, and brain. Mutations in KCNJ13 gene are associated with snowflake vitreoretinal degeneration.

实验方案

Product Specific Protocols
WB protocol for Kir7.1 antibody 12657-1-APDownload protocol
IHC protocol for Kir7.1 antibody 12657-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanIHC

Acta Biochim Biophys Sin (Shanghai)

Identification of lipid metabolism-related genes as prognostic indicators in papillary thyroid cancer.

Authors - Shishuai Wen
View Article