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验证数据展示

  • WB analysis of human brain using 12657-1-AP

    WB analysis of human brain using 12657-1-AP

    human brain tissue were subjected to SDS PAGE followed by western blot with 12657-1-AP (Kir7.1 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • IHC staining of human small intestine using 12657-1-AP

    IHC staining of human small intestine using 12657-1-AP

    Immunohistochemical analysis of paraffin-embedded human small intestine tissue slide using 12657-1-AP (Kir7.1 antibody at dilution of 1:50 (under 10x lens).

  • IHC staining of human small intestine using 12657-1-AP

    IHC staining of human small intestine using 12657-1-AP

    Immunohistochemical analysis of paraffin-embedded human small intestine tissue slide using 12657-1-AP (Kir7.1 antibody at dilution of 1:50 (under 40x lens).

Kir7.1 Polyclonal antibody

Kir7.1 Polyclonal Antibody for WB, IHC, ELISA
Cat No. 12657-1-AP

产品说明书

引用文献(1)

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, IHC, ELISA

别名

KCNJ13, KIR1.4, Kir7.1, SVD

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inhuman brain tissue, human kidney tissue
Positive IHC detected inhuman small intestine tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:200-1:1000
Immunohistochemistry (IHC)IHC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

IHCSee 1 publications below

产品信息

12657-1-AP targets Kir7.1 in WB, IHC, ELISA applications and shows reactivity with human samples.

经测试应用 WB, IHC, ELISA Application Description
文献引用应用IHC
经测试反应性 human
文献引用反应性human
免疫原 Kir7.1 fusion protein Ag3327 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 potassium inwardly-rectifying channel, subfamily J, member 13
别名 KCNJ13, KIR1.4, Kir7.1, SVD
计算分子量 360 aa, 43 kDa
观测分子量 43 kDa
GenBank蛋白编号BC037290
基因名称 Kir7.1
Gene ID (NCBI) 3769
RRIDAB_2130488
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDO60928
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Kir7.1, encoded by KCNJ13 gene, is a member of the inwardly rectifying potassium channel family of proteins. Kir7.1 is a low-conductance channel. At mRNA level, Kir7.1 is predominantly expressed in small intestine and also expressed in stomach, kidney, and brain. Mutations in KCNJ13 gene are associated with snowflake vitreoretinal degeneration.

实验方案

Product Specific Protocols
WB protocol for Kir7.1 antibody 12657-1-APDownload protocol
IHC protocol for Kir7.1 antibody 12657-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanIHC

Acta Biochim Biophys Sin (Shanghai)

Identification of lipid metabolism-related genes as prognostic indicators in papillary thyroid cancer.

Authors - Shishuai Wen
View Article