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验证数据展示

  • IHC staining of mouse brain using 83257-5-RR (same clone as 83257-5-PBS)

    IHC staining of mouse brain using 83257-5-RR (same clone as 83257-5-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 83257-5-RR (KCNJ15 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 83257-5-PBS in a different storage buffer formulation.

  • FC experiment of HepG2 using 83257-5-RR (same clone as 83257-5-PBS)

    FC experiment of HepG2 using 83257-5-RR (same clone as 83257-5-PBS)

    1x10^6 HepG2 cells were intracellularly stained with 0.25 ug KCNJ15 Recombinant antibody (83257-5-RR, Clone:240141C3) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.25 ug Control Antibody (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 83257-5-PBS in a different storage buffer formulation.

  • WB analysis of A549 using 83257-5-RR (same clone as 83257-5-PBS)

    WB analysis of A549 using 83257-5-RR (same clone as 83257-5-PBS)

    A549 cells were subjected to SDS PAGE followed by western blot with 83257-5-RR (KCNJ15 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 83257-5-PBS in a different storage buffer formulation.

  • Affinity and Kinetic Characterization of 83257-5-RR

    Affinity and Kinetic Characterization of 83257-5-RR

    Biolayer interferometry (BLl) kinetic assays of 83257-5-RR against Human KCNJ15 were performed. The affinity constant is 0.221 nM.

KCNJ15 Recombinant antibody, PBS Only

KCNJ15 Uni-rAbTM Recombinant Antibody for WB, IHC, ELISA
Cat No. 83257-5-PBS

产品说明书

CloneNo. 240141C3

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, IHC, ELISA

别名

KIR4.2, KIR1.3, KCNJ14, IRKK, ATP-sensitive inward rectifier potassium channel 15

缓冲液配方:  PBS Only
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格: 

-/ -

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产品信息

83257-5-PBS targets KCNJ15 in WB, IHC, ELISA applications and shows reactivity with human samples.

经测试应用 WB, IHC, ELISA Application Description
经测试反应性 human
免疫原 KCNJ15 fusion protein Ag34655 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 potassium inwardly-rectifying channel, subfamily J, member 15
别名 KIR4.2, KIR1.3, KCNJ14, IRKK, ATP-sensitive inward rectifier potassium channel 15
计算分子量 375 aa, 43 kDa
观测分子量43 kDa
GenBank蛋白编号BC013327
基因名称 KCNJ15
Gene ID (NCBI) 3772
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purfication
UNIPROT IDQ99712
储存缓冲液 PBS only , pH 7.3
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

KCNJ15, also named as KIR1.3, KIR4.2, and IRKK, is a multi-pass membrane protein belonging to the inwardly-rectifying potassium channel (KIR) family. KIR channels perform functions as diverse as the regulation of resting membrane potential, maintenance of potassium ions homeostasis, control of heart rate and hormone secretion. Cloned from human kidney, the KCNJ15 gene is localized on chromosome 21 in the Down syndrome chromosome region 1 (DCR1) and has been identified as a type 2 diabetes-associated risk gene. The gene is most readily expressed in the pancreas and kidney and less in the lung. (PMID: 8995301; 9299242; 20085713)