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KCNJ15 Recombinant antibody

KCNJ15 Uni-rAbTM Recombinant Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

CloneNo.

240141C3

Cat no : 83257-5-RR

Print datasheet

Synonyms

KIR4.2, KIR1.3, KCNJ14, IRKK, ATP-sensitive inward rectifier potassium channel 15

验证数据展示

  • IHC staining of mouse brain using 83257-5-RR

    IHC staining of mouse brain using 83257-5-RR

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 83257-5-RR (KCNJ15 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • FC experiment of HepG2 using 83257-5-RR

    FC experiment of HepG2 using 83257-5-RR

    1x10^6 HepG2 cells were intracellularly stained with 0.25 ug KCNJ15 Recombinant antibody (83257-5-RR, Clone:240141C3) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.25 ug Control Antibody (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

  • WB analysis of A549 using 83257-5-RR

    WB analysis of A549 using 83257-5-RR

    A549 cells were subjected to SDS PAGE followed by western blot with 83257-5-RR (KCNJ15 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Affinity and Kinetic Characterization of 83257-5-RR

    Affinity and Kinetic Characterization of 83257-5-RR

    Biolayer interferometry (BLl) kinetic assays of 83257-5-RR against Human KCNJ15 were performed. The affinity constant is 0.221 nM.

经过测试的应用

Positive WB detected inA549 cells
Positive IHC detected inmouse brain tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive FC (Intra) detected inHepG2 cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

The immunogen of 83257-5-RR is KCNJ15 Fusion Protein expressed in E. coli.

Tested Applications WB, IHC, ELISA Application Description
Tested Reactivity human
Immunogen KCNJ15 fusion protein Ag34655 种属同源性预测
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Full Name potassium inwardly-rectifying channel, subfamily J, member 15
Synonyms KIR4.2, KIR1.3, KCNJ14, IRKK, ATP-sensitive inward rectifier potassium channel 15
Calculated Molecular Weight 375 aa, 43 kDa
Observed Molecular Weight43 kDa
GenBank Accession NumberBC013327
Gene Symbol KCNJ15
Gene ID (NCBI) 3772
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
UNIPROT IDQ99712
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

KCNJ15, also named as KIR1.3, KIR4.2, and IRKK, is a multi-pass membrane protein belonging to the inwardly-rectifying potassium channel (KIR) family. KIR channels perform functions as diverse as the regulation of resting membrane potential, maintenance of potassium ions homeostasis, control of heart rate and hormone secretion. Cloned from human kidney, the KCNJ15 gene is localized on chromosome 21 in the Down syndrome chromosome region 1 (DCR1) and has been identified as a type 2 diabetes-associated risk gene. The gene is most readily expressed in the pancreas and kidney and less in the lung. (PMID: 8995301; 9299242; 20085713)

实验方案

Product Specific Protocols
WB protocol for KCNJ15 antibody 83257-5-RRDownload protocol
IHC protocol for KCNJ15 antibody 83257-5-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols