验证数据展示
经过测试的应用
| Positive WB detected in | human blood tissue |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
发表文章中的应用
| WB | See 5 publications below |
| IF | See 2 publications below |
产品信息
60276-1-Ig targets KCNN4 in WB, IF, ELISA applications and shows reactivity with human, mouse, rat samples.
| 经测试应用 | WB, ELISA Application Description |
| 文献引用应用 | WB, IF |
| 经测试反应性 | human, mouse, rat |
| 文献引用反应性 | human, mouse, rat |
| 免疫原 | KCNN4 fusion protein Ag19774 种属同源性预测 |
| 宿主/亚型 | Mouse / IgG2a |
| 抗体类别 | Monoclonal |
| 产品类型 | Antibody |
| 全称 | potassium intermediate/small conductance calcium-activated channel, subfamily N, member 4 |
| 别名 | hIKCa1, hKCa4, hSK4, IK1, IKCA1, KCa3.1, KCA4, KCNN4, Putative Gardos channel, SK4, SKCa 4, SKCa4 |
| 计算分子量 | 427 aa, 48 kDa |
| 观测分子量 | 48 kDa |
| GenBank蛋白编号 | BC015337 |
| 基因名称 | KCNN4 |
| Gene ID (NCBI) | 3783 |
| RRID | AB_2881395 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Protein A purification |
| UNIPROT ID | O15554 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
The intermediate conductance calcium-activated potassium channel KCNN4, also known as KCa3.1, IK1, or SK4, is a member of the potassium channel KCNN family. KCNN4 is expressed in T cells, mast cells, macrophages, erythrocytes, vascular smooth muscle cells, airway smooth muscle cells, intestinal and airway epithelial cells (PMID: 19681865; 21493782). Functionally, KCNN4 plays a key role in calcium-dependent cell functions, such as proliferation, activation, and migration, in a broad range of cell types (PMID: 21493782).
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for KCNN4 antibody 60276-1-Ig | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
J Inflamm Res KCa3.1 Inhibition of Macrophages Suppresses Inflammatory Response Leading to Endothelial Damage in a Cell Model of Kawasaki Disease. | ||
Mol Med Rep M2 macrophage‑derived exosomes alleviate KCa3.1 channel expression in rapidly paced HL‑1 myocytes via the NF‑κB (p65)/STAT3 signaling pathway | ||
Nat Commun Phospholipid scrambling induced by an ion channel/metabolite transporter complex | ||
J Hematol Oncol The LINC00623/NAT10 signaling axis promotes pancreatic cancer progression by remodeling ac4C modification of mRNA | ||
Cell Death Dis IKCa channels control breast cancer metabolism including AMPK-driven autophagy |