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×
×
Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0

验证数据展示

  • WB analysis using 29977-1-AP (same clone as 29977-1-PBS)

    WB analysis using 29977-1-AP (same clone as 29977-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 29977-1-AP (KDM4A antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 29977-1-PBS in a different storage buffer formulation.

  • FC experiment of A549 using 29977-1-AP (same clone as 29977-1-PBS)

    FC experiment of A549 using 29977-1-AP (same clone as 29977-1-PBS)

    1X10^6 A549 cells were intracellularly stained with 0.4 ug Anti-Human KDM4A (29977-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Control Antibody. Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011). This data was developed using the same antibody clone with 29977-1-PBS in a different storage buffer formulation.

  • IP experiment of MCF-7 using 29977-1-AP (same clone as 29977-1-PBS)

    IP experiment of MCF-7 using 29977-1-AP (same clone as 29977-1-PBS)

    IP result of anti-KDM4A (IP:29977-1-AP, 4ug; Detection:29977-1-AP 1:1500) with MCF-7 cells lysate 2120 ug. This data was developed using the same antibody clone with 29977-1-PBS in a different storage buffer formulation.

KDM4A Polyclonal antibody, PBS Only

KDM4A Polyclonal Antibody for WB, FC (Intra), IP, Indirect ELISA
Cat No. 29977-1-PBS

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse

应用

WB, FC (Intra), IP, Indirect ELISA

别名

缓冲液配方:  PBS Only
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格: 

-/ -

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产品信息

29977-1-PBS targets KDM4A in WB, FC (Intra), IP, Indirect ELISA applications and shows reactivity with human, mouse samples.

经测试应用 WB, FC (Intra), IP, Indirect ELISA Application Description
经测试反应性 human, mouse
免疫原 KDM4A fusion protein Ag31992 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 lysine (K)-specific demethylase 4A
别名
计算分子量 1064 aa, 121 kDa
观测分子量130-150 kDa
GenBank蛋白编号BC002558
基因名称 KDM4A
Gene ID (NCBI) 9682
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDO75164
储存缓冲液 PBS only , pH 7.3
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

KDM4A also named as JHDM3A, JMJD2, or KIAA0677 is a 1066 amino acid protein, which contains one JmjC domain, two Tudor domains and two PHD-type zinc fingers. KDM4A localizes in nucleus and belongs to the JHDM3 histone demethylase family. KDM4A is histone de-methylase that specifically demethylates Lys 9 and Lys 36 residues of Histone H3, thereby playing a central role in histone code. JMJD2A demethylation of lysine residues will generate formaldehyde and succinate. KDM4A also participates in transcriptional repression of ASCL2 and E2F-responsive promoters via the recruitment of histone deacetylases and NCOR1, respectively. JMJD2A is a ubiquitously expressed nuclear protein. KDM4A exists as two isoforms. The short isoform of KDM4A in which the N-terminal demethylase domains has been deleted. The molecular weight of short isoform is 55Da.