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KDM4A Polyclonal antibody, PBS Only

KDM4A Polyclonal Antibody for WB, FC (Intra), IP, Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, FC (Intra), IP, Indirect ELISA

Conjugate

Unconjugated

Cat no : 29977-1-PBS

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Synonyms

验证数据展示

  • WB analysis using 29977-1-AP (same clone as 29977-1-PBS)

    WB analysis using 29977-1-AP (same clone as 29977-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 29977-1-AP (KDM4A antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 29977-1-PBS in a different storage buffer formulation.

  • FC experiment of A549 using 29977-1-AP (same clone as 29977-1-PBS)

    FC experiment of A549 using 29977-1-AP (same clone as 29977-1-PBS)

    1X10^6 A549 cells were intracellularly stained with 0.4 ug Anti-Human KDM4A (29977-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Control Antibody. Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011). This data was developed using the same antibody clone with 29977-1-PBS in a different storage buffer formulation.

  • IP experiment of MCF-7 using 29977-1-AP (same clone as 29977-1-PBS)

    IP experiment of MCF-7 using 29977-1-AP (same clone as 29977-1-PBS)

    IP result of anti-KDM4A (IP:29977-1-AP, 4ug; Detection:29977-1-AP 1:1500) with MCF-7 cells lysate 2120 ug. This data was developed using the same antibody clone with 29977-1-PBS in a different storage buffer formulation.

产品信息

29977-1-PBS targets KDM4A in WB, FC (Intra), IP, Indirect ELISA applications and shows reactivity with human, mouse samples.

Tested Applications WB, FC (Intra), IP, Indirect ELISA Application Description
Tested Reactivity human, mouse
Immunogen KDM4A fusion protein Ag31992 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name lysine (K)-specific demethylase 4A
Synonyms
Calculated Molecular Weight 1064 aa, 121 kDa
Observed Molecular Weight130-150 kDa
GenBank Accession NumberBC002558
Gene Symbol KDM4A
Gene ID (NCBI) 9682
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDO75164
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

KDM4A also named as JHDM3A, JMJD2, or KIAA0677 is a 1066 amino acid protein, which contains one JmjC domain, two Tudor domains and two PHD-type zinc fingers. KDM4A localizes in nucleus and belongs to the JHDM3 histone demethylase family. KDM4A is histone de-methylase that specifically demethylates Lys 9 and Lys 36 residues of Histone H3, thereby playing a central role in histone code. JMJD2A demethylation of lysine residues will generate formaldehyde and succinate. KDM4A also participates in transcriptional repression of ASCL2 and E2F-responsive promoters via the recruitment of histone deacetylases and NCOR1, respectively. JMJD2A is a ubiquitously expressed nuclear protein. KDM4A exists as two isoforms. The short isoform of KDM4A in which the N-terminal demethylase domains has been deleted. The molecular weight of short isoform is 55Da.