验证数据展示
经过测试的应用
| Positive WB detected in | HeLa cells, Jurkat cells, MCF-7 cells |
| Positive IF/ICC detected in | HeLa cells |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:5000 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
14426-1-AP targets KDM5C in WB, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.
| 经测试应用 | WB, IF/ICC, ELISA Application Description |
| 文献引用应用 | WB, IF, IP |
| 经测试反应性 | human, mouse, rat |
| 文献引用反应性 | human, mouse |
| 免疫原 | KDM5C fusion protein Ag5791 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | lysine (K)-specific demethylase 5C |
| 别名 | Lysine-specific demethylase 5C, Jumonji/ARID domain-containing protein 1C, JARID1C, EC:1.14.11.67, DXS1272E |
| 计算分子量 | 176 kDa |
| 观测分子量 | 171-180 kDa |
| GenBank蛋白编号 | BC054499 |
| 基因名称 | KDM5C |
| Gene ID (NCBI) | 8242 |
| RRID | AB_10837073 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | P41229 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
KDM5C belongs to the ARID protein family that harbors conserved DNA-binding motifs and acts as a histone H3 lysine 4 demethylase, suggesting a potential role in epigenetic regulation during development, cell differentiation (PMID:21575681). KDM5C protein is homologous to three other proteins of the human JARID1 family (JARID1A, JARID1B and JARID1C). This family shares several evolutionarily conserved domains, including the catalytic JmjC domain, JmjN domain, Arid/Bright domain, C5HC2 zinc-finger domain and two plant homeodomains (PHD), a motif that binds methyl-lysine residues (PMID:17320160,17320161).
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for KDM5C antibody 14426-1-AP | Download protocol |
| IF protocol for KDM5C antibody 14426-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Cancer Res KDM5C-mediated recruitment of BRD4 to chromatin regulates enhancer activation and BET inhibitor sensitivity | ||
Neurol Genet Novel Variations in the KDM5C Gene Causing X-Linked Intellectual Disability. | ||
Nat Metab Myc-mediated SDHA acetylation triggers epigenetic regulation of gene expression and tumorigenesis. | ||
EMBO Rep Targeting the transcription factor YY1 is synthetic lethal with loss of the histone demethylase KDM5C |