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验证数据展示

  • IHC staining of human placenta using 83049-4-RR (same clone as 83049-4-PBS)

    IHC staining of human placenta using 83049-4-RR (same clone as 83049-4-PBS)

    Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 83049-4-RR (KDR antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 83049-4-PBS in a different storage buffer formulation.

  • IHC staining of human placenta using 83049-4-RR (same clone as 83049-4-PBS)

    IHC staining of human placenta using 83049-4-RR (same clone as 83049-4-PBS)

    Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 83049-4-RR (KDR antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 83049-4-PBS in a different storage buffer formulation.

  • WB analysis of human placenta using 83049-4-RR (same clone as 83049-4-PBS)

    WB analysis of human placenta using 83049-4-RR (same clone as 83049-4-PBS)

    human placenta tissue was subjected to SDS PAGE followed by western blot with 83049-4-RR (KDR antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 83049-4-PBS in a different storage buffer formulation.

  • FC experiment of HUVEC using 83049-4-RR (same clone as 83049-4-PBS)

    FC experiment of HUVEC using 83049-4-RR (same clone as 83049-4-PBS)

    1x10^6 HUVEC cells were surface stained with 0.25 ug VEGF Receptor 2 Recombinant antibody (83049-4-RR, Clone:230238D7) and PE-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were not fixed. This data was developed using the same antibody clone with 83049-4-PBS in a different storage buffer formulation.

  • Affinity and Kinetic Characterization of 83049-4-RR

    Affinity and Kinetic Characterization of 83049-4-RR

    Biolayer interferometry (BLl) kinetic assays of 83049-4-RR against Human KDR were performed. The affinity constant is 4.71 nM.

VEGFR2/KDR Recombinant antibody, PBS Only

VEGFR2/KDR Uni-rAbTM Recombinant Antibody for WB, IHC, FC, Indirect ELISA
Cat No. 83049-4-PBS

产品说明书

CloneNo. 230238D7

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, IHC, FC, Indirect ELISA

别名

KDR, VEGFR2, Protein-tyrosine kinase receptor flk-1, FLK-1, EC:2.7.10.1

缓冲液配方:  PBS Only
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格: 

-/ -

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产品信息

83049-4-PBS targets VEGFR2/KDR in WB, IHC, FC, Indirect ELISA applications and shows reactivity with human samples.

经测试应用 WB, IHC, FC, Indirect ELISA Application Description
经测试反应性 human
免疫原 Recombinant protein 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 kinase insert domain receptor (a type III receptor tyrosine kinase)
别名 KDR, VEGFR2, Protein-tyrosine kinase receptor flk-1, FLK-1, EC:2.7.10.1
计算分子量152kd
观测分子量150 kDa, 230 kDa
GenBank蛋白编号NM_002253
基因名称 KDR
Gene ID (NCBI) 3791
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purification
UNIPROT IDP35968-1
储存缓冲液 PBS only , pH 7.3
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

KDR, also named as VEGFR-2, FLK1 and CD309, is a receptor for VEGF or VEGFC. KDR which belongs to the protein kinase superfamily, has a tyrosine-protein kinase activity. The VEGF-kinase ligand/receptor signaling system plays a key role in vascular development and regulation of vascular permeability. In case of HIV-1 infection, the interaction with extracellular viral Tat protein seems to enhance angiogenesis in Kaposi's sarcoma lesions. KDR functions as the main mediator of VEGF-induced endothelial proliferation, survival, migration, tubular morphogenesis and sprouting. Mutations of this gene are implicated in infantile capillary hemangiomas.