验证数据展示
经过测试的应用
| Positive WB detected in | human placenta tissue |
| Positive IHC detected in | human placenta tissue, human rectal cancer tissue, human tonsillitis tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive FC detected in | HUVEC cells |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| Immunohistochemistry (IHC) | IHC : 1:200-1:800 |
| Flow Cytometry (FC) | FC : 0.25 ug per 10^6 cells in a 100 µl suspension |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
发表文章中的应用
| WB | See 1 publications below |
产品信息
83049-4-RR targets VEGFR2/KDR in WB, IHC, FC, ELISA applications and shows reactivity with human samples.
| 经测试应用 | WB, IHC, FC, ELISA Application Description |
| 文献引用应用 | WB |
| 经测试反应性 | human |
| 文献引用反应性 | human |
| 免疫原 | Recombinant protein 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Recombinant |
| 产品类型 | Antibody |
| 全称 | kinase insert domain receptor (a type III receptor tyrosine kinase) |
| 别名 | KDR, VEGFR2, Protein-tyrosine kinase receptor flk-1, FLK-1, EC:2.7.10.1 |
| 计算分子量 | 152kd |
| 观测分子量 | 150 kDa, 230 kDa |
| GenBank蛋白编号 | NM_002253 |
| 基因名称 | KDR |
| Gene ID (NCBI) | 3791 |
| RRID | AB_3670774 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Protein A purification |
| UNIPROT ID | P35968-1 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
KDR, also named as VEGFR-2, FLK1 and CD309, is a receptor for VEGF or VEGFC. KDR which belongs to the protein kinase superfamily, has a tyrosine-protein kinase activity. The VEGF-kinase ligand/receptor signaling system plays a key role in vascular development and regulation of vascular permeability. In case of HIV-1 infection, the interaction with extracellular viral Tat protein seems to enhance angiogenesis in Kaposi's sarcoma lesions. KDR functions as the main mediator of VEGF-induced endothelial proliferation, survival, migration, tubular morphogenesis and sprouting. Mutations of this gene are implicated in infantile capillary hemangiomas.
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for VEGFR2/KDR antibody 83049-4-RR | Download protocol |
| IHC protocol for VEGFR2/KDR antibody 83049-4-RR | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
