• All
  • Primary Antibodies
  • Conjugated Antibodies for IF
  • Conjugated Antibodies for FC
  • Secondary Antibodies
  • Antibody Labeling KitsNew
  • ELISA Kits
  • IHC Kits
  • Magnetic Cell Separation Kits
  • Cytokines & Growth Factors
  • Neutralizing/activating Antibodies
  • Nanobody-based Reagents
  • Accessory Products and Kits
  • Fusion Proteins
×
×
Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0
  • Featured Product
  • KD/KO Validated

KEAP1 Monoclonal antibody, PBS Only

KEAP1 Monoclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA

Host / Isotype

Mouse / IgA

Reactivity

human, rat, mouse

Applications

WB, IHC, IF/ICC, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

4G10H9

Cat no : 60027-1-PBS

Print datasheet

Synonyms

Cytosolic inhibitor of Nrf2, INrf2, KEAP1, Kelch like protein 19, KIAA0132, KLHL19

验证数据展示

  • WB analysis of 4T1 using 60027-1-Ig (same clone as 60027-1-PBS)

    WB analysis of 4T1 using 60027-1-Ig (same clone as 60027-1-PBS)

    4T1 cells were subjected to SDS PAGE followed by western blot with 60027-1-Ig (KEAP1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 60027-1-PBS in a different storage buffer formulation.

  • WB analysis of HeLa using 60027-1-Ig (same clone as 60027-1-PBS)

    WB analysis of HeLa using 60027-1-Ig (same clone as 60027-1-PBS)

    HeLa cells were subjected to SDS PAGE followed by western blot with 60027-1-Ig (KEAP1 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 60027-1-PBS in a different storage buffer formulation.

  • IHC staining of human pancreas cancer using 60027-1-Ig (same clone as 60027-1-PBS)

    IHC staining of human pancreas cancer using 60027-1-Ig (same clone as 60027-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human pancreas cancer using 60027-1-Ig(KEAP1 antibody) at dilution of 1:50 (under 40x lens). This data was developed using the same antibody clone with 60027-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with KEAP1 antibody included
  • WB analysis of MCF-7 using 60027-1-Ig (same clone as 60027-1-PBS)

    WB analysis of MCF-7 using 60027-1-Ig (same clone as 60027-1-PBS)

    MCF-7 cells were subjected to SDS PAGE followed by western blot with 60027-1-Ig (KEAP1 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 60027-1-PBS in a different storage buffer formulation.

  • WB analysis of Jurkat using 60027-1-Ig (same clone as 60027-1-PBS)

    WB analysis of Jurkat using 60027-1-Ig (same clone as 60027-1-PBS)

    Jurkat cells were subjected to SDS PAGE followed by western blot with 60027-1-Ig (KEAP1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 60027-1-PBS in a different storage buffer formulation.

  • IF Staining of HeLa using 60027-1-Ig (same clone as 60027-1-PBS)

    IF Staining of HeLa using 60027-1-Ig (same clone as 60027-1-PBS)

    Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using KEAP1 antibody (60027-1-Ig, Clone: 4G10H9 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 60027-1-PBS in a different storage buffer formulation.

Mouse monoclonal antibodies of IgA isotype can be detected with "anti-mouse IgG (H+L)" secondary antibodies.

Planning an IHC experiment? We recommend our IHCeasy KEAP1 Ready-To-Use IHC Kit. KEAP1 primary antibody included.

产品信息

60027-1-PBS targets KEAP1 in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human, rat, mouse samples.

Tested Applications WB, IHC, IF/ICC, Indirect ELISA Application Description
Tested Reactivity human, rat, mouse
Immunogen KEAP1 fusion protein Ag0779 种属同源性预测
Host / Isotype Mouse / IgA
Class Monoclonal
Type Antibody
Full Name kelch-like ECH-associated protein 1
Synonyms Cytosolic inhibitor of Nrf2, INrf2, KEAP1, Kelch like protein 19, KIAA0132, KLHL19
Calculated Molecular Weight 624 aa, 70 kDa
Observed Molecular Weight60~70 kDa
GenBank Accession NumberBC002930
Gene Symbol KEAP1
Gene ID (NCBI) 9817
Conjugate Unconjugated
Form Liquid
Purification MethodThiophilic affinity chromatograph
UNIPROT IDQ14145
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

Kelch-like ECH-associated protein 1 (KEAP1) is a negative regulator of nuclear factor erythroid 2-related factor 2 (Nrf2), a transcription factor governing the antioxidant response.

 

What is the molecular weight of KEAP1 protein? Are there any isoforms of KEAP1?

The molecular weight of KEAP1 protein is 70 kDa. The KEAP1 gene gives rise only to protein isoforms, but mutations of KEAP1 protein have been found in various cancer types.

 

What is the subcellular localization of KEAP1?

KEAP1 resides in the cytoplasm, where it binds to Nrf2, targeting it for degradation and preventing translocation of Nrf2 to the nucleus.

 

How does KEAP1 control Nrf2 levels? Is KEAP1 post-translationally modified?

KEAP1 is rich in reactive cysteine residues, whose thiol groups play a role in binding to CUL3 and the polyubiquitination of Nrf2, which leads to degradation of Nrf2 via the proteasome system. During oxidative stress, electrophiles and reactive oxygen species (ROS) modify the KEAP1 thiol groups, reducing the affinity of KEAP1 to CUL3 and the stabilization of Nrf2. Nrf2 then translocates to the nucleus, where it binds to the antioxidant responsive elements (AREs) and induces the expression of antioxidant proteins (PMID: 16354693).

 

How to measure oxidative stress using KEAP1 and Nrf2 proteins as a readout

Under basal conditions (unstressed cells), a detectable KEAP1 protein level is observed. Oxidative stress modifies KEAP1 protein activity by increasing the Nrf2 protein levels. This can be measured, for example, using western blotting (PMID: 27697860). KEAP1 protein levels are not altered by oxidative stress.

 

What is the role of the KEAP1-Nrf2 pathway in health and disease?

The KEAP-Nrf2 pathway plays a vital role in redox homeostasis and cryoprotection. Inhibition of KEAP1 activity leads to the activation of Nrf2 and increase the response to oxidative stress and anti-inflammatory effects (PMID: 29717933). The activation of Nrf2 can be beneficial in the case of metabolic diseases, such as diabetes, as well as neurodegenerative diseases such as Parkinson's and Alzheimer's diseases. However, the increased activation of Nrf2 is also known to promote tumor growth and metastasis. Mutations in both KEAP1 and Nrf2 were found in various solid tumor types.