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KEAP1 Monoclonal antibody

KEAP1 Monoclonal Antibody for WB, ELISA
Cat No. 60027-1-Ig

产品说明书

CloneNo. 4G10H9

宿主/亚型

Mouse / IgA

种属反应性

human, mouse, rat and More (2)

应用

WB, ELISA and More (4)

4G10H9, KEAP 1, Kelch-like ECH-associated protein 1, KIAA0132, KLHL19

缓冲液配方:  PBS and Azide
PBS and Azide
PBS Only
偶联物:  Unconjugated
Unconjugated
CoraLite® Plus 488
规格: 

-/ -


经过测试的应用

Positive WB detected in4T1 cells, HeLa cells, HSC-T6 cells, Jurkat cells, MCF-7 cells, NIH/3T3 cells

Mouse monoclonal antibodies of IgA isotype can be detected with "anti-mouse IgG (H+L)" secondary antibodies.

Planning an IHC experiment? We recommend our IHCeasy KEAP1 Ready-To-Use IHC Kit. KEAP1 primary antibody included.
Planning an IF experiment? We recommend our CoraLite® Plus 488 conjugated versions of this antibody.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:1000-1:4000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

60027-1-Ig targets KEAP1 in WB, IHC, IF, IP, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, ELISA Application Description
文献引用应用WB, IHC, IF, IP, CoIP
经测试反应性 human, mouse, rat
文献引用反应性human, mouse, rat, pig, chicken
免疫原 KEAP1 fusion protein Ag0779 种属同源性预测
宿主/亚型 Mouse / IgA
抗体类别 Monoclonal
产品类型 Antibody
全称 kelch-like ECH-associated protein 1
别名 4G10H9, KEAP 1, Kelch-like ECH-associated protein 1, KIAA0132, KLHL19
计算分子量 624 aa, 70 kDa
观测分子量60~70 kDa
GenBank蛋白编号BC002930
基因名称 KEAP1
Gene ID (NCBI) 9817
RRIDAB_2132623
偶联类型 Unconjugated
形式 Liquid
纯化方式Caprylic acid/ammonium sulfate precipitation
UNIPROT IDQ14145
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Kelch-like ECH-associated protein 1 (KEAP1) is a negative regulator of nuclear factor erythroid 2-related factor 2 (Nrf2), a transcription factor governing the antioxidant response.

 

What is the molecular weight of KEAP1 protein? Are there any isoforms of KEAP1?

The molecular weight of KEAP1 protein is 70 kDa. The KEAP1 gene gives rise only to protein isoforms, but mutations of KEAP1 protein have been found in various cancer types.

 

What is the subcellular localization of KEAP1?

KEAP1 resides in the cytoplasm, where it binds to Nrf2, targeting it for degradation and preventing translocation of Nrf2 to the nucleus.

 

How does KEAP1 control Nrf2 levels? Is KEAP1 post-translationally modified?

KEAP1 is rich in reactive cysteine residues, whose thiol groups play a role in binding to CUL3 and the polyubiquitination of Nrf2, which leads to degradation of Nrf2 via the proteasome system. During oxidative stress, electrophiles and reactive oxygen species (ROS) modify the KEAP1 thiol groups, reducing the affinity of KEAP1 to CUL3 and the stabilization of Nrf2. Nrf2 then translocates to the nucleus, where it binds to the antioxidant responsive elements (AREs) and induces the expression of antioxidant proteins (PMID: 16354693).

 

How to measure oxidative stress using KEAP1 and Nrf2 proteins as a readout

Under basal conditions (unstressed cells), a detectable KEAP1 protein level is observed. Oxidative stress modifies KEAP1 protein activity by increasing the Nrf2 protein levels. This can be measured, for example, using western blotting (PMID: 27697860). KEAP1 protein levels are not altered by oxidative stress.

 

What is the role of the KEAP1-Nrf2 pathway in health and disease?

The KEAP-Nrf2 pathway plays a vital role in redox homeostasis and cryoprotection. Inhibition of KEAP1 activity leads to the activation of Nrf2 and increase the response to oxidative stress and anti-inflammatory effects (PMID: 29717933). The activation of Nrf2 can be beneficial in the case of metabolic diseases, such as diabetes, as well as neurodegenerative diseases such as Parkinson's and Alzheimer's diseases. However, the increased activation of Nrf2 is also known to promote tumor growth and metastasis. Mutations in both KEAP1 and Nrf2 were found in various solid tumor types.



实验方案

Product Specific Protocols
WB protocol for KEAP1 antibody 60027-1-IgDownload protocol
IHC protocol for KEAP1 antibody 60027-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
mouseIF

ACS Nano

Neutrophil Nanovesicle Protects against Experimental Autoimmune Encephalomyelitis through Enhancing Myelin Clearance by Microglia

Authors - Shishi Shen
humanWB,IF

J Clin Invest

FAM117B promotes gastric cancer growth and drug resistance by targeting the KEAP1/NRF2 signaling pathway

Authors - Yunjiang Zhou
humanIHC

Mol Cell

KEAP1 E3 ligase-mediated downregulation of NF-kappaB signaling by targeting IKKbeta.

Authors - Dung-Fang Lee
mouseIF

Aging Cell

Chaperone-mediated autophagy degrades Keap1 and promotes Nrf2-mediated antioxidative response.

Authors - Lin Zhu
humanWB,IP,IF

Cell Death Dis

ΔNp63α promotes radioresistance in esophageal squamous cell carcinoma through the PLEC-KEAP1-NRF2 feedback loop

Authors - Jin Tao
humanWB

EMBO Rep

Chemical and genetic control of IFNγ-induced MHCII expression.

Authors - Ruud H Wijdeven
  • KD Validated
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