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验证数据展示

  • WB analysis using 24933-1-AP (same clone as 24933-1-PBS)

    WB analysis using 24933-1-AP (same clone as 24933-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 24933-1-AP (KEPI antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 24933-1-PBS in a different storage buffer formulation.

  • WB analysis of HeLa using 24933-1-AP (same clone as 24933-1-PBS)

    WB analysis of HeLa using 24933-1-AP (same clone as 24933-1-PBS)

    WB result of KEPI antibody (24933-1-AP; 1:3000; incubated at room temperature for 1.5 hours) with sh-Control and sh-KEPI transfected HeLa cells. This data was developed using the same antibody clone with 24933-1-PBS in a different storage buffer formulation.

  • Featured Product
  • KD/KO验证

KEPI Polyclonal antibody, PBS Only

KEPI Polyclonal Antibody for WB, Indirect ELISA
Cat No. 24933-1-PBS

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, Indirect ELISA

别名

缓冲液配方:  PBS Only
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格: 

-/ -

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产品信息

24933-1-PBS targets KEPI in WB, Indirect ELISA applications and shows reactivity with human samples.

经测试应用 WB, Indirect ELISA Application Description
经测试反应性 human
免疫原 KEPI fusion protein Ag20645 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 protein phosphatase 1, regulatory (inhibitor) subunit 14C
别名
计算分子量 18 kDa
观测分子量20-22 kDa
GenBank蛋白编号BC015773
基因名称 PPP1R14C
Gene ID (NCBI) 81706
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ8TAE6
储存缓冲液 PBS only , pH 7.3
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

PPP1R14C, found as a morphine‐regulated brain gene, encodes protein phosphatase 1 regulatory inhibitor subunit 14C, and is identified as a potent inhibitor of PP1. Horvath and colleagues reported that PPP1R14C inhibited the release of neurotransmitters and promoted neuronal exocytosis by retaining synaptosome associated protein 25 (SNAP25) at a phosphorylated state. Moreover, overexpression of PPP1R14C increased the phosphorylation of RB transcriptional co‐repressor 1 (RB1) to protect leukemic cells from chemotherapy.