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CoraLite® Plus 488-conjugated KGA-Specific Monoclonal antibody

KGA-Specific Monoclonal Antibody for WB, IF-P

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse, rat

Applications

WB, IF-P

Conjugate

CoraLite® Plus 488 Fluorescent Dye

CloneNo.

1B11A11

Cat no : CL488-66265

Print datasheet

Synonyms

AAD20, DKFZp686O15119, FLJ10358, GAC, GLS, GLS1, glutaminase, K glutaminase, KGA-Specific, KIAA0838, L glutamine amidohydrolase

验证数据展示

  • WB analysis using CL488-66265

    WB analysis using CL488-66265

    Various lysates were subjected to SDS PAGE followed by western blot with CL488-66265 (KGA-Specific antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • IF Staining of mouse brain using CL488-66265

    IF Staining of mouse brain using CL488-66265

    Immunofluorescent analysis of (4% PFA) fixed mouse brain tissue using CoraLite® Plus 488 KGA-Specific antibody (CL488-66265, Clone: 1B11A11 ) at dilution of 1:200.

经过测试的应用

Positive WB detected inNIH/3T3 cells, mouse brain tissue
Positive IF-P detected inmouse brain tissue
Planning an IHC experiment? We recommend our IHCeasy KGA/GAC Ready-To-Use IHC Kit. KGA/GAC primary antibody included.
For other applications, we recommend the unconjugated version of this antibody, 66265-1-Ig

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunofluorescence (IF)-PIF-P : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL488-66265 targets KGA-Specific in WB, IF-P applications and shows reactivity with human, mouse, rat samples.

Tested Applications WB, IF-P Application Description
Tested Reactivity human, mouse, rat
Immunogen KGA-Specific fusion protein Ag24227 种属同源性预测
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Full Name glutaminase
Synonyms AAD20, DKFZp686O15119, FLJ10358, GAC, GLS, GLS1, glutaminase, K glutaminase, KGA-Specific, KIAA0838, L glutamine amidohydrolase
Calculated Molecular Weight 669 aa, 73 kDa
Observed Molecular Weight 65 kDa
GenBank Accession NumberBC038507
Gene Symbol GLS
Gene ID (NCBI) 2744
Conjugate CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission Maxima Wavelengths493 nm / 522 nm
Form Liquid
Purification MethodProtein G purification
UNIPROT IDO94925
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

GLS, also named as GLS1 and KIAA0838, belongs to the glutaminase family. It catalyzes the first reaction in the primary pathway for the renal catabolism of glutamine. Glutaminase-, glutamate-, and taurine-immunoreactive neurons develop neurofibrillary tangles in Alzheimer's disease.The glutaminase band in AA/C1 cells is more intense than in HT29 cells, in accordance with measurements of glutaminase activity, and had the same molecular mass of approx. 63 kDa. The bands for both cell lines are clearly different in size from both rat liver glutaminase (58 kDa) and rat kidney glutaminase (65 kDa)(PMID: 12408749). It also reveals a molecular weight of 83-84 kDa as a phosphate-dependent glutaminase(PMID: 447624;7512428). It has 3 isoforms produced by alternative splicing named as KGA, GAM, GAC. This antibody is specific to KGA.

实验方案

Product Specific Protocols
WB protocol for CL Plus 488 KGA-Specific antibody CL488-66265Download protocol
IF protocol for CL Plus 488 KGA-Specific antibody CL488-66265Download protocol
Standard Protocols
Click here to view our Standard Protocols