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KHDC1 Polyclonal antibody

KHDC1 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

Cat no : 23443-1-AP

Print datasheet

Synonyms

NDG1, KHDC 1, KH homology domain-containing protein 1, C6orf148, C6orf147

验证数据展示

  • IHC staining of human stomach cancer using 23443-1-AP

    IHC staining of human stomach cancer using 23443-1-AP

    Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 23443-1-AP (KHDC1 antibody) at dilution of 1:100 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of human stomach cancer using 23443-1-AP

    IHC staining of human stomach cancer using 23443-1-AP

    Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 23443-1-AP (KHDC1 antibody) at dilution of 1:100 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • WB analysis of L02 using 23443-1-AP

    WB analysis of L02 using 23443-1-AP

    L02 cells were subjected to SDS PAGE followed by western blot with 23443-1-AP (KHDC1 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • IF Staining of L02 using 23443-1-AP

    IF Staining of L02 using 23443-1-AP

    Immunofluorescent analysis of L02 cells using 23443-1-AP (KHDC1 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

  • IHC staining of human stomach using 23443-1-AP

    IHC staining of human stomach using 23443-1-AP

    Immunohistochemical analysis of paraffin-embedded human normal stomach slide using 23443-1-AP (KHDC1 antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

经过测试的应用

Positive WB detected inL02 cells
Positive IHC detected inhuman stomach cancer tissue, human liver tissue, human stomach tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inL02 cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

23443-1-AP targets KHDC1 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human samples.

Tested Applications WB, IHC, IF/ICC, ELISA Application Description
Tested Reactivity human
Immunogen KHDC1 fusion protein Ag20115 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name KH homology domain containing 1
Synonyms NDG1, KHDC 1, KH homology domain-containing protein 1, C6orf148, C6orf147
Calculated Molecular Weight 237 aa, 27 kDa
Observed Molecular Weight 35-40 kDa
GenBank Accession NumberBC022080
Gene Symbol KHDC1
Gene ID (NCBI) 80759
RRIDAB_2879280
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen Affinity purified
UNIPROT IDQ4VXA5
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

KHDC1, also named as C6orf148, belongs to the KHDC1 family. KHDC1 has two isoforms with calculated MW 27 kDa and 18 kDa. It's a membrane protein. We got 35-40 kDa in our WB detection.

实验方案

Product Specific Protocols
WB protocol for KHDC1 antibody 23443-1-APDownload protocol
IHC protocol for KHDC1 antibody 23443-1-APDownload protocol
IF protocol for KHDC1 antibody 23443-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols