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验证数据展示

  • IP experiment of HeLa using 82931-2-RR

    IP experiment of HeLa using 82931-2-RR

    IP result of anti-KHSRP (IP:82931-2-RR, 4ug; Detection:82931-2-RR 1:2500) with HeLa cells lysate 1050 ug.

  • FC experiment of U-251 using 82931-2-RR

    FC experiment of U-251 using 82931-2-RR

    1x10^6 U-251 cells were intracellularly stained with 0.25 ug Anti-Human KHSRP (82931-2-RR, Clone:230189C12) and APC-Conjugated Goat Anti-Rabbit IgG(H+L) (red), or 0.25 ug Rabbit IgG control Rabbit PolyAb (30000-0-AP) (blue). Cells were fixed and permeabilized with True-Nuclear Transcription Factor Buffer Set.

  • Affinity and Kinetic Characterization of 82931-2-RR

    Affinity and Kinetic Characterization of 82931-2-RR

    Biolayer interferometry (BLl) kinetic assays of 82931-2-RR against Human KHSRP were performed. The affinity constant is 1.42 nM.

KHSRP Recombinant antibody

KHSRP Uni-rAbTM Recombinant Antibody for FC (Intra), IP, ELISA
Cat No. 82931-2-RR

产品说明书

CloneNo. 230189C12

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

FC (Intra), IP, ELISA

别名

KH type-splicing regulatory protein, FUSE-binding protein 2, FUBP2, FBP2, Far upstream element-binding protein 2

缓冲液配方:  PBS and Azide
PBS and Azide
PBS Only
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive IP detected inHeLa cells
Positive FC (Intra) detected inU-251 cells

推荐稀释比

应用推荐稀释比
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

82931-2-RR targets KHSRP in FC (Intra), IP, ELISA applications and shows reactivity with human samples.

经测试应用 FC (Intra), IP, ELISA Application Description
经测试反应性 human
免疫原 KHSRP fusion protein Ag33890 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 KH-type splicing regulatory protein
别名 KH type-splicing regulatory protein, FUSE-binding protein 2, FUBP2, FBP2, Far upstream element-binding protein 2
计算分子量73 kDa
GenBank蛋白编号NM_003685
基因名称 KHSRP
Gene ID (NCBI) 8570
RRIDAB_3670670
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purification
UNIPROT IDQ92945
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

KHSRP, also named as FUBP2, KSRP, and p75, belongs to the KHSRP family. It binds to the dendritic targeting element and may play a role in mRNA trafficking. KHSRP is a part of a ternary complex that binds to the downstream control sequence (DCS) of the pre-mRNA. It mediates exon inclusion in transcripts that are subject to tissue-specific alternative splicing. KHSRP may interact with single-stranded DNA from the far-upstream element (FUSE). May activate gene expression. It is involved in the degradation of inherently unstable mRNAs that contain AU-rich elements (AREs) in their 3'-UTR, possibly by recruiting degradation machinery to ARE-containing mRNAs.

实验方案

Product Specific Protocols
IP protocol for KHSRP antibody 82931-2-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols