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验证数据展示

  • IP experiment of HeLa using 82931-2-RR

    IP experiment of HeLa using 82931-2-RR

    IP result of anti-KHSRP (IP:82931-2-RR, 4ug; Detection:82931-2-RR 1:2500) with HeLa cells lysate 1050 ug.

  • FC experiment of U-251 using 82931-2-RR

    FC experiment of U-251 using 82931-2-RR

    1x10^6 U-251 cells were intracellularly stained with 0.25 ug Anti-Human KHSRP (82931-2-RR, Clone:230189C12) and APC-Conjugated Goat Anti-Rabbit IgG(H+L) (red), or 0.25 ug Rabbit IgG control Rabbit PolyAb (30000-0-AP) (blue). Cells were fixed and permeabilized with True-Nuclear Transcription Factor Buffer Set.

  • Affinity and Kinetic Characterization of 82931-2-RR

    Affinity and Kinetic Characterization of 82931-2-RR

    Biolayer interferometry (BLl) kinetic assays of 82931-2-RR against Human KHSRP were performed. The affinity constant is 1.42 nM.

KHSRP Recombinant antibody

KHSRP Uni-rAbTM Recombinant Antibody for FC (Intra), IP, ELISA
Cat No. 82931-2-RR
CloneNo. 230189C12

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

FC (Intra), IP, ELISA

Synonyms

KH type-splicing regulatory protein, FUSE-binding protein 2, FUBP2, FBP2, Far upstream element-binding protein 2

Formulation:  PBS and Azide
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Unconjugated
Size: 

-/ -

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经过测试的应用

Positive IP detected inHeLa cells
Positive FC (Intra) detected inU-251 cells

推荐稀释比

ApplicationDilution
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

82931-2-RR targets KHSRP in FC (Intra), IP, ELISA applications and shows reactivity with human samples.

Tested Applications FC (Intra), IP, ELISA Application Description
Tested Reactivity human
Immunogen KHSRP fusion protein Ag33890 种属同源性预测
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Full Name KH-type splicing regulatory protein
Synonyms KH type-splicing regulatory protein, FUSE-binding protein 2, FUBP2, FBP2, Far upstream element-binding protein 2
Calculated Molecular Weight73 kDa
GenBank Accession NumberNM_003685
Gene Symbol KHSRP
Gene ID (NCBI) 8570
RRIDAB_3670670
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDQ92945
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

KHSRP, also named as FUBP2, KSRP, and p75, belongs to the KHSRP family. It binds to the dendritic targeting element and may play a role in mRNA trafficking. KHSRP is a part of a ternary complex that binds to the downstream control sequence (DCS) of the pre-mRNA. It mediates exon inclusion in transcripts that are subject to tissue-specific alternative splicing. KHSRP may interact with single-stranded DNA from the far-upstream element (FUSE). May activate gene expression. It is involved in the degradation of inherently unstable mRNAs that contain AU-rich elements (AREs) in their 3'-UTR, possibly by recruiting degradation machinery to ARE-containing mRNAs.

实验方案

Product Specific Protocols
IP protocol for KHSRP antibody 82931-2-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols