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验证数据展示

  • WB analysis using 82931-3-RR

    WB analysis using 82931-3-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 82931-3-RR (KHSRP antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • IP experiment of HeLa using 82931-3-RR

    IP experiment of HeLa using 82931-3-RR

    IP result of anti-KHSRP (IP:82931-3-RR, 4ug; Detection:82931-3-RR 1:2500) with HeLa cells lysate 1050 ug.

  • IHC staining of human colon using 82931-3-RR

    IHC staining of human colon using 82931-3-RR

    Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 82931-3-RR (KHSRP antibody) at dilution of 1:800 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IF Staining of HeLa using 82931-3-RR

    IF Staining of HeLa using 82931-3-RR

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using KHSRP antibody (82931-3-RR, Clone: 230189B2 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).

  • Affinity and Kinetic Characterization of 82931-3-RR

    Affinity and Kinetic Characterization of 82931-3-RR

    Biolayer interferometry (BLl) kinetic assays of 82931-3-RR against Human KHSRP were performed. The affinity constant is 0.991 nM.

KHSRP Recombinant antibody

KHSRP Recombinant Antibody for WB, IHC, IF/ICC, IP, ELISA
Cat No. 82931-3-RR

产品说明书

CloneNo. 230189B2

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, IF/ICC, IP, ELISA

别名

KH type-splicing regulatory protein, FUSE-binding protein 2, FUBP2, FBP2, Far upstream element-binding protein 2

缓冲液配方:  PBS and Azide
PBS and Azide
PBS Only
偶联物:  Unconjugated
Unconjugated
CoraLite® Plus 488
规格: 

-/ -

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经过测试的应用

Positive WB detected inHeLa cells, HEK-293T cells, Jurkat cells, mouse spleen tissue, mouse brain tissue, rat brain tissue
Positive IP detected inHeLa cells
Positive IHC detected inhuman colon tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHeLa cells

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:5000-1:50000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:400-1:1600
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

82931-3-RR targets KHSRP in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, IF/ICC, IP, ELISA Application Description
经测试反应性 human, mouse, rat
免疫原 KHSRP fusion protein Ag33890 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 KH-type splicing regulatory protein
别名 KH type-splicing regulatory protein, FUSE-binding protein 2, FUBP2, FBP2, Far upstream element-binding protein 2
计算分子量73 kDa
观测分子量75 kDa
GenBank蛋白编号NM_003685
基因名称 KHSRP
Gene ID (NCBI) 8570
RRIDAB_3670671
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purification
UNIPROT IDQ92945
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

KHSRP, also named as FUBP2, KSRP, and p75, belongs to the KHSRP family. It binds to the dendritic targeting element and may play a role in mRNA trafficking. KHSRP is a part of a ternary complex that binds to the downstream control sequence (DCS) of the pre-mRNA. It mediates exon inclusion in transcripts that are subject to tissue-specific alternative splicing. KHSRP may interact with single-stranded DNA from the far-upstream element (FUSE). May activate gene expression. It is involved in the degradation of inherently unstable mRNAs that contain AU-rich elements (AREs) in their 3'-UTR, possibly by recruiting degradation machinery to ARE-containing mRNAs.

实验方案

Product Specific Protocols
WB protocol for KHSRP antibody 82931-3-RRDownload protocol
IHC protocol for KHSRP antibody 82931-3-RRDownload protocol
IF protocol for KHSRP antibody 82931-3-RRDownload protocol
IP protocol for KHSRP antibody 82931-3-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols