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验证数据展示

  • IF Staining of A549 using CL488-82931-3

    IF Staining of A549 using CL488-82931-3

    Immunofluorescent analysis of (4% PFA) fixed A549 cells using CoraLite® Plus 488 KHSRP antibody (CL488-82931-3, Clone: 230189B2 ) at dilution of 1:200.

  • FC experiment of A549 using CL488-82931-3

    FC experiment of A549 using CL488-82931-3

    1x10^6 A549 cells were intracellularly stained with 0.4 ug CoraLite® Plus 488-conjugated KHSRP Recombinant antibody (CL488-82931-3, Clone:230189B2)(red), or 0.4 ug CoraLite® Plus 488-conjugated Rabbit IgG control Rabbit PolyAb (CL488-30000) (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

CoraLite® Plus 488-conjugated KHSRP Recombinant antibody

KHSRP Uni-rAbTM Recombinant Antibody for IF/ICC, FC (Intra)
Cat No. CL488-82931-3

产品说明书

CloneNo. 230189B2

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

IF/ICC, FC (Intra)

别名

KH type-splicing regulatory protein, FUSE-binding protein 2, FUBP2, FBP2, Far upstream element-binding protein 2

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  CoraLite® Plus 488
规格: 

-/ -

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经过测试的应用

Positive IF/ICC detected inA549 cells
Positive FC (Intra) detected inA549 cells
For other applications, we recommend the unconjugated version of this antibody, 82931-3-PBS

推荐稀释比

应用推荐稀释比
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL488-82931-3 targets KHSRP in IF/ICC, FC (Intra) applications and shows reactivity with human, mouse, rat samples.

经测试应用 IF/ICC, FC (Intra) Application Description
经测试反应性 human, mouse, rat
免疫原 KHSRP fusion protein Ag33890 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 KH-type splicing regulatory protein
别名 KH type-splicing regulatory protein, FUSE-binding protein 2, FUBP2, FBP2, Far upstream element-binding protein 2
计算分子量73 kDa
观测分子量75 kDa
GenBank蛋白编号NM_003685
基因名称 KHSRP
Gene ID (NCBI) 8570
RRIDAB_3673141
偶联类型 CoraLite® Plus 488 Fluorescent Dye
最大激发/发射波长493 nm / 522 nm
形式 Liquid
纯化方式Protein A purification
UNIPROT IDQ92945
储存缓冲液 PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA , pH 7.3
储存条件Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

KHSRP, also named as FUBP2, KSRP, and p75, belongs to the KHSRP family. It binds to the dendritic targeting element and may play a role in mRNA trafficking. KHSRP is a part of a ternary complex that binds to the downstream control sequence (DCS) of the pre-mRNA. It mediates exon inclusion in transcripts that are subject to tissue-specific alternative splicing. KHSRP may interact with single-stranded DNA from the far-upstream element (FUSE). May activate gene expression. It is involved in the degradation of inherently unstable mRNAs that contain AU-rich elements (AREs) in their 3'-UTR, possibly by recruiting degradation machinery to ARE-containing mRNAs.

实验方案

Product Specific Protocols
IF protocol for CL Plus 488 KHSRP antibody CL488-82931-3Download protocol
FC protocol for CL Plus 488 KHSRP antibody CL488-82931-3Download protocol
Standard Protocols
Click here to view our Standard Protocols