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验证数据展示

  • FC experiment of HepG2 using CL594-21129

    FC experiment of HepG2 using CL594-21129

    1X10^6 HepG2 cells were intracellularly stained with 0.4 ug CoraLite®594 Anti-Human KIAA1199 (CL594-21129) (red), or 0.4 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

  • Featured Product
  • KD/KO Validated

CoraLite®594-conjugated KIAA1199 / CEMIP Polyclonal antibody

KIAA1199 / CEMIP Polyclonal Antibody for FC (Intra)
Cat No. CL594-21129

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Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

FC (Intra)

Synonyms

KIAA1199, CEMIP, Protein KIAA1199, HYBID, Hyaluronan binding protein involved in hyaluronan depolymerization

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  CoraLite®594

Size: 
100 μL

¥3200/ 100 μL

浓度: 1000 ug/ml

库存：现货

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经过测试的应用

Positive FC (Intra) detected inHepG2 cells
For other applications, we recommend the unconjugated version of this antibody, 21129-1-AP

推荐稀释比

ApplicationDilution
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL594-21129 targets KIAA1199 / CEMIP in FC (Intra) applications and shows reactivity with human, mouse, rat samples.

Tested Applications FC (Intra) Application Description
Tested Reactivity human, mouse, rat
Immunogen KIAA1199 / CEMIP fusion protein Ag15527 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name KIAA1199 / CEMIP
Synonyms KIAA1199, CEMIP, Protein KIAA1199, HYBID, Hyaluronan binding protein involved in hyaluronan depolymerization
Calculated Molecular Weight 1361 aa, 153 kDa
Observed Molecular Weight 150 kDa
GenBank Accession NumberBC020256
Gene Symbol KIAA1199
Gene ID (NCBI) 57214
RRIDAB_2919859
Conjugate CoraLite®594 Fluorescent Dye
Excitation/Emission Maxima Wavelengths588 nm / 604 nm
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ8WUJ3
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

KIAA1199, also known as CEMIP, was first discovered as an inner-ear protein with genetic mutations linked to nonsyndromic hearing loss. KIAA1199 is involved in hyaluronan degradation. Studies have shown that KIAA1199 is overexpressed in many types of cancers, suggesting a critical role of KIAA1199 in cancer progression.

实验方案

Product Specific Protocols
FC protocol for CL594 KIAA1199 / CEMIP antibody CL594-21129Download protocol
Standard Protocols
Click here to view our Standard Protocols