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KIAA1429 Monoclonal antibody, PBS Only

KIAA1429 Monoclonal Antibody for Indirect ELISA, WB

Host / Isotype

Mouse / IgG1

Reactivity

mouse, human

Applications

Indirect ELISA, WB

Conjugate

Unconjugated

CloneNo.

3F1A4

Cat no : 68235-1-PBS

Print datasheet

Synonyms

VIRMA, MSTP054, 3F1A4

验证数据展示

  • WB analysis using 68235-1-Ig (same clone as 68235-1-PBS)

    WB analysis using 68235-1-Ig (same clone as 68235-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 68235-1-Ig (KIAA1429 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68235-1-PBS in a different storage buffer formulation.

  • FC experiment of MCF-7 using 68235-1-Ig (same clone as 68235-1-PBS)

    FC experiment of MCF-7 using 68235-1-Ig (same clone as 68235-1-PBS)

    1X10^6 MCF-7 cells were intracellularly stained with 0.4 ug Anti-Human KIAA1429 (68235-1-Ig, Clone:3F1A4) and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 68235-1-PBS in a different storage buffer formulation.

  • WB analysis using 68235-1-Ig (same clone as 68235-1-PBS)

    WB analysis using 68235-1-Ig (same clone as 68235-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 68235-1-Ig (KIAA1429 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68235-1-PBS in a different storage buffer formulation.

Planning an IHC experiment? We recommend our IHCeasy VIRMA/KIAA1429 Ready-To-Use IHC Kit. VIRMA/KIAA1429 primary antibody included.

产品信息

68235-1-PBS targets KIAA1429 in Indirect ELISA, WB applications and shows reactivity with mouse, human samples.

Tested Applications Indirect ELISA, WB Application Description
Tested Reactivity mouse, human
Immunogen KIAA1429 fusion protein Ag22449 种属同源性预测
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Full Name KIAA1429
Synonyms VIRMA, MSTP054, 3F1A4
Calculated Molecular Weight 1812 aa, 202 kDa
Observed Molecular Weight200-210 kDa
GenBank Accession NumberBC113380
Gene Symbol KIAA1429
Gene ID (NCBI) 25962
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
UNIPROT IDQ69YN4
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

VIRMA (KIAA1429) is a key component of m6A methyltransferase (writer) complex that include METTL3, METTL14, WTAP, VIRMA, HAKAI, ZC3H13, and RBM15. VIRMA mediates methylation in the 3'UTR and around the stop codon, thus affecting alternative polyadenylation. VIRMA has been reported to act as oncogenic factor in breast cancer and liver cancer.