验证数据展示
经过测试的应用
Positive WB detected in | LNCaP cells, NIH/3T3 cells, HeLa cells, HepG2 cells, HEK-293 cells, Jurkat cells, K-562 cells, 4T1 cells |
Positive FC (Intra) detected in | MCF-7 cells |
推荐稀释比
Application | Dilution |
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Western Blot (WB) | WB : 1:5000-1:50000 |
Flow Cytometry (FC) (INTRA) | FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
产品信息
68235-1-Ig targets KIAA1429 in WB, FC (Intra), ELISA applications and shows reactivity with human, mouse samples.
Tested Applications | WB, FC (Intra), ELISA Application Description |
Tested Reactivity | human, mouse |
Immunogen | KIAA1429 fusion protein Ag22449 种属同源性预测 |
Host / Isotype | Mouse / IgG1 |
Class | Monoclonal |
Type | Antibody |
Full Name | KIAA1429 |
Synonyms | VIRMA, MSTP054, 3F1A4 |
Calculated Molecular Weight | 1812 aa, 202 kDa |
Observed Molecular Weight | 200-210 kDa |
GenBank Accession Number | BC113380 |
Gene Symbol | KIAA1429 |
Gene ID (NCBI) | 25962 |
RRID | AB_2935322 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Protein G purification |
UNIPROT ID | Q69YN4 |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
VIRMA (KIAA1429) is a key component of m6A methyltransferase (writer) complex that include METTL3, METTL14, WTAP, VIRMA, HAKAI, ZC3H13, and RBM15. VIRMA mediates methylation in the 3'UTR and around the stop codon, thus affecting alternative polyadenylation. VIRMA has been reported to act as oncogenic factor in breast cancer and liver cancer.
实验方案
Product Specific Protocols | |
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WB protocol for KIAA1429 antibody 68235-1-Ig | Download protocol |
FC protocol for KIAA1429 antibody 68235-1-Ig | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |