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验证数据展示

  • FC experiment of MCF-7 using CL488-68235

    FC experiment of MCF-7 using CL488-68235

    1X10^6 MCF-7 cells were intracellularly stained with 0.8 ug CoraLite® Plus 488 Anti-Human KIAA1429 (CL488-68235, Clone:3F1A4) (red), or 0.8 ug CoraLite® Plus 488 Mouse IgG1 Isotype Control (MOPC-21) (CL488-65124, Clone: MOPC-21) (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

CoraLite® Plus 488-conjugated KIAA1429 Monoclonal antibody

KIAA1429 Monoclonal Antibody for FC (Intra)
Cat No. CL488-68235

Print datasheet

CloneNo. 3F1A4

Host / Isotype

Mouse / IgG1

Reactivity

Human, mouse

Applications

FC (Intra)

Synonyms

KIAA1429, VIRMA, 3F1A4, MSTP054

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  CoraLite® Plus 488
Size: 

-/ -

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经过测试的应用

Positive FC (Intra) detected inMCF-7 cells
Positive FC detected inMCF-7 cells
Planning an IHC experiment? We recommend our IHCeasy VIRMA/KIAA1429 Ready-To-Use IHC Kit. VIRMA/KIAA1429 primary antibody included.
For other applications, we recommend the unconjugated version of this antibody, 68235-1-Ig

推荐稀释比

ApplicationDilution
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.80 ug per 10^6 cells in a 100 µl suspension
Flow Cytometry (FC)FC : 0.80 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL488-68235 targets KIAA1429 in FC (Intra) applications and shows reactivity with Human, mouse samples.

Tested Applications FC (Intra) Application Description
Tested Reactivity Human, mouse
Immunogen KIAA1429 fusion protein Ag22449 种属同源性预测
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Full Name KIAA1429
Synonyms KIAA1429, VIRMA, 3F1A4, MSTP054
Calculated Molecular Weight 1812 aa, 202 kDa
Observed Molecular Weight200-210 kDa
GenBank Accession NumberBC113380
Gene Symbol KIAA1429
Gene ID (NCBI) 25962
RRIDAB_3084451
Conjugate CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission Maxima Wavelengths493 nm / 522 nm
Form Liquid
Purification MethodProtein G purification
UNIPROT IDQ69YN4
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

VIRMA (KIAA1429) is a key component of m6A methyltransferase (writer) complex that include METTL3, METTL14, WTAP, VIRMA, HAKAI, ZC3H13, and RBM15. VIRMA mediates methylation in the 3'UTR and around the stop codon, thus affecting alternative polyadenylation. VIRMA has been reported to act as oncogenic factor in breast cancer and liver cancer.

实验方案

Product Specific Protocols
FC protocol for CL Plus 488 KIAA1429 antibody CL488-68235Download protocol
Standard Protocols
Click here to view our Standard Protocols