验证数据展示
经过测试的应用
Positive FC (Intra) detected in | MCF-7 cells |
Positive FC detected in | MCF-7 cells |
推荐稀释比
应用 | 推荐稀释比 |
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Flow Cytometry (FC) (INTRA) | FC (INTRA) : 0.80 ug per 10^6 cells in a 100 µl suspension |
Flow Cytometry (FC) | FC : 0.80 ug per 10^6 cells in a 100 µl suspension |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
产品信息
CL488-68235 targets KIAA1429 in FC (Intra) applications and shows reactivity with Human, mouse samples.
经测试应用 | FC (Intra) Application Description |
经测试反应性 | Human, mouse |
免疫原 | KIAA1429 fusion protein Ag22449 种属同源性预测 |
宿主/亚型 | Mouse / IgG1 |
抗体类别 | Monoclonal |
产品类型 | Antibody |
全称 | KIAA1429 |
别名 | KIAA1429, VIRMA, 3F1A4, MSTP054 |
计算分子量 | 1812 aa, 202 kDa |
观测分子量 | 200-210 kDa |
GenBank蛋白编号 | BC113380 |
基因名称 | KIAA1429 |
Gene ID (NCBI) | 25962 |
RRID | AB_3084451 |
偶联类型 | CoraLite® Plus 488 Fluorescent Dye |
最大激发/发射波长 | 493 nm / 522 nm |
形式 | Liquid |
纯化方式 | Protein G purification |
UNIPROT ID | Q69YN4 |
储存缓冲液 | PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA , pH 7.3 |
储存条件 | Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
VIRMA (KIAA1429) is a key component of m6A methyltransferase (writer) complex that include METTL3, METTL14, WTAP, VIRMA, HAKAI, ZC3H13, and RBM15. VIRMA mediates methylation in the 3'UTR and around the stop codon, thus affecting alternative polyadenylation. VIRMA has been reported to act as oncogenic factor in breast cancer and liver cancer.
实验方案
Product Specific Protocols | |
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FC protocol for CL Plus 488 KIAA1429 antibody CL488-68235 | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |