验证数据展示
经过测试的应用
| Positive WB detected in | HEK-293 cells, Jurkat cells |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:6000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
66748-1-Ig targets KIDINS220 in WB, ELISA applications and shows reactivity with Human samples.
| 经测试应用 | WB, ELISA Application Description |
| 经测试反应性 | Human |
| 免疫原 | KIDINS220 fusion protein Ag17624 种属同源性预测 |
| 宿主/亚型 | Mouse / IgG2b |
| 抗体类别 | Monoclonal |
| 产品类型 | Antibody |
| 全称 | kinase D-interacting substrate, 220kDa |
| 别名 | ARMS, KIAA1250, KIDINS220 |
| 计算分子量 | 1672 aa, 187 kDa |
| 观测分子量 | 200 kDa |
| GenBank蛋白编号 | BC130610 |
| 基因名称 | KIDINS220 |
| Gene ID (NCBI) | 57498 |
| RRID | AB_2882095 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Protein A purification |
| UNIPROT ID | Q9ULH0 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
Kidins220 (Kinase D-interacting substrate 220 kD) is a crucial mediator of signal transduction in neural tissues. Also it named ARMS(Ankyrin Repeat-Rich Membrane Spanning) due to the presence of ankyrin repeats and transmembrane domains together with other binding motifs. It is a multidomain transmembrane protein that not only transduces signaling mediated by the receptor tyrosine kinase (RTK), but also regulates the stability of cytoskeletal network through a number of cytoskeleton-associated proteins, including Septin5, MAPs , and stathmin family members. KIDINS220 exists some isoforms with MV 194 kDa, 185 kDa, 115 kDa, and 60 kDa.
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for KIDINS220 antibody 66748-1-Ig | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |