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验证数据展示

  • WB analysis of HepG2 using 27210-1-AP

    WB analysis of HepG2 using 27210-1-AP

    HepG2 cells were subjected to SDS PAGE followed by western blot with 27210-1-AP (KIF13A antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • IHC staining of human liver cancer using 27210-1-AP

    IHC staining of human liver cancer using 27210-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 27210-1-AP (KIF13A antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

KIF13A Polyclonal antibody

KIF13A Polyclonal Antibody for WB, IHC, ELISA
Cat No. 27210-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, IHC, ELISA

别名

KIF13A, kinesin family member 13A, Kinesin like protein KIF13A, Kinesin like protein RBKIN, RBKIN

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inHepG2 cells
Positive IHC detected inhuman liver cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

27210-1-AP targets KIF13A in WB, IHC, ELISA applications and shows reactivity with human samples.

经测试应用 WB, IHC, ELISA Application Description
经测试反应性 human
免疫原 KIF13A fusion protein Ag24356 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 kinesin family member 13A
别名 KIF13A, kinesin family member 13A, Kinesin like protein KIF13A, Kinesin like protein RBKIN, RBKIN
计算分子量 1805 aa, 202 kDa
观测分子量 202 kDa
GenBank蛋白编号BC127115
基因名称 KIF13A
Gene ID (NCBI) 63971
RRIDAB_2880802
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ9H1H9
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for KIF13A antibody 27210-1-APDownload protocol
IHC protocol for KIF13A antibody 27210-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols