验证数据展示
经过测试的应用
| Positive IF/ICC detected in | HeLa cells |
Planning an IHC experiment? We recommend our IHCeasy KIF20A Ready-To-Use IHC Kit. KIF20A primary antibody included.
For other applications, we recommend the unconjugated version of this antibody, 67190-1-Ig
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
发表文章中的应用
| IF | See 1 publications below |
产品信息
CL594-67190 targets KIF20A in IF/ICC applications and shows reactivity with Human, Rat, Mouse samples.
| 经测试应用 | IF/ICC Application Description |
| 文献引用应用 | IF |
| 经测试反应性 | Human, Rat, Mouse |
| 免疫原 | KIF20A fusion protein Ag8916 种属同源性预测 |
| 宿主/亚型 | Mouse / IgG1 |
| 抗体类别 | Monoclonal |
| 产品类型 | Antibody |
| 全称 | kinesin family member 20A |
| 别名 | FLJ21151, GG10_2, KIF20A, kinesin family member 20A, Kinesin like protein KIF20A, MKLP2, RAB6KIFL, Rabkinesin 6 |
| 计算分子量 | 890 aa, 100 kDa |
| 观测分子量 | 100 kDa |
| GenBank蛋白编号 | BC012999 |
| 基因名称 | KIF20A |
| Gene ID (NCBI) | 10112 |
| RRID | AB_2883645 |
| 偶联类型 | CoraLite®594 Fluorescent Dye |
| 最大激发/发射波长 | 588 nm / 604 nm |
| 形式 | Liquid |
| 纯化方式 | Protein G purification |
| UNIPROT ID | O95235 |
| 储存缓冲液 | PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA , pH 7.3 |
| 储存条件 | Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
KIF20A, also known as RAB6KIFL or MKlp2, is a kinesin originally identified as a binding partner for the rab6 GTPase involved in protein transport at the Golgi apparatus. KIF20A belongs to a large family of motor proteins that accumulate in mitotic cells and is required for normal cell division. KIF20A is thought to be involved in carcinogenesis. In normal tissues KIF20A is almost undetectable while it is overexpressed in various cancers. KIF20A has been identified as a novel promising candidate for anticancer immunotherapy.
实验方案
| Product Specific Protocols | |
|---|---|
| IF protocol for CL594 KIF20A antibody CL594-67190 | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
