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KIF2B Polyclonal antibody

KIF2B Polyclonal Antibody for WB, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IP, ELISA

Conjugate

Unconjugated

Cat no : 13711-1-AP

Print datasheet

Synonyms

KIF2B, kinesin family member 2B, Kinesin like protein KIF2B

验证数据展示

  • WB analysis of mouse brain using 13711-1-AP

    WB analysis of mouse brain using 13711-1-AP

    mouse brain tissue were subjected to SDS PAGE followed by western blot with 13711-1-AP (KIF2B antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • IP experiment of mouse testis using 13711-1-AP

    IP experiment of mouse testis using 13711-1-AP

    IP result of anti-KIF2B (IP:13711-1-AP, 4ug; Detection:13711-1-AP 1:1000) with mouse testis tissue lysate 4000ug.

经过测试的应用

Positive WB detected inmouse brain tissue, mouse testis tissue
Positive IP detected inmouse testis tissue

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

13711-1-AP targets KIF2B in WB, IP, ELISA applications and shows reactivity with human, mouse samples.

Tested Applications WB, IP, ELISA Application Description
Tested Reactivity human, mouse
Immunogen KIF2B fusion protein Ag4648 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name kinesin family member 2B
Synonyms KIF2B, kinesin family member 2B, Kinesin like protein KIF2B
Calculated Molecular Weight 673 aa, 76 kDa
Observed Molecular Weight 80 kDa
GenBank Accession NumberBC033802
Gene Symbol KIF2B
Gene ID (NCBI) 84643
RRIDAB_2131885
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ8N4N8
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

KIF2B belongs to the TRAFAC class myosin-kinesin ATPase superfamily, Kinesin family and MCAK/KIF2 subfamily. KIF2B is evidently required for spindle assembly, chromosome movement and cytokinesis (PMID: 17538014). The molecular weight of KIF2B is 76 kDa. It plays an important role in chromosome congression (PMID: 23891108).

实验方案

Product Specific Protocols
WB protocol for KIF2B antibody 13711-1-APDownload protocol
IP protocol for KIF2B antibody 13711-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols