验证数据展示
经过测试的应用
| Positive IP detected in | mouse testis tissue |
| Positive IF/ICC detected in | HeLa cells |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:20-1:200 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
12139-1-AP targets KIF2C in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.
| 经测试应用 | IP, IF, ELISA Application Description |
| 文献引用应用 | WB, IHC, IF |
| 经测试反应性 | human, mouse, rat |
| 文献引用反应性 | human, mouse |
| 免疫原 | KIF2C fusion protein Ag2786 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | kinesin family member 2C |
| 别名 | KIF2C, kinesin family member 2C, Kinesin like protein 6, Kinesin like protein KIF2C, KNSL6, MCAK |
| 计算分子量 | 725 aa, 81 kDa |
| 观测分子量 | 81 kDa |
| GenBank蛋白编号 | BC014924 |
| 基因名称 | KIF2C |
| Gene ID (NCBI) | 11004 |
| RRID | AB_2877829 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | Q99661 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
实验方案
| Product Specific Protocols | |
|---|---|
| IF protocol for KIF2C antibody 12139-1-AP | Download protocol |
| IP protocol for KIF2C antibody 12139-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Elife KIF2C regulates synaptic plasticity and cognition in mice through dynamic microtubule depolymerization. | ||
J Physiol Ablation of KIF2C in Purkinje cells impairs metabotropic glutamate receptor trafficking and motor coordination in male mice | ||
Front Cell Dev Biol Integrative Analysis of Biomarkers Through Machine Learning Identifies Stemness Features in Colorectal Cancer. | ||
Front Oncol Novel prognostic biomarkers in nasopharyngeal carcinoma unveiled by mega-data bioinformatics analysis | ||
Eur J Med Res Potential 'anti-cancer' effects of esketamine on proliferation, apoptosis, migration and invasion in esophageal squamous carcinoma cells | ||
Carcinogenesis Systematically characterize the clinical and biological significances of 1p19q genes in 1p/19q non-codeletion glioma. |