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  • Featured Product
  • KD/KO Validated

KIF5B Recombinant antibody

KIF5B Uni-rAbTM Recombinant Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

CloneNo.

2B18

Cat no : 82485-1-RR

Print datasheet

Synonyms

KNS1, KNS, KINH, Kinesin-1 heavy chain, KIF 5B

验证数据展示

  • WB analysis using 82485-1-RR

    WB analysis using 82485-1-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 82485-1-RR (KIF5B antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • WB analysis of HeLa using 82485-1-RR

    WB analysis of HeLa using 82485-1-RR

    WB result of KIF5B antibody (82485-1-RR; 1:6000; incubated at room temperature for 1.5 hours) with sh-Control and sh-KIF5B transfected HeLa cells.

  • IHC staining of human ovarian cancer using 82485-1-RR

    IHC staining of human ovarian cancer using 82485-1-RR

    Immunohistochemical analysis of paraffin-embedded human ovarian cancer slide using 82485-1-RR (KIF5B antibody) at dilution of 1:1000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with KIF5B antibody included
  • IF Staining of HepG2 using 82485-1-RR

    IF Staining of HepG2 using 82485-1-RR

    Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using KIF5B antibody (82485-1-RR, Clone: 2B18 ) at dilution of 1:200 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002).

经过测试的应用

Positive WB detected inHeLa cells, HepG2 cells, A549 cells, Jurkat cells, mouse heart tissue, mouse brain tissue, rat brain tissue
Positive IHC detected inhuman ovarian cancer
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHepG2 cells
Planning an IHC experiment? We recommend our IHCeasy KIF5B Ready-To-Use IHC Kit. KIF5B primary antibody included.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunohistochemistry (IHC)IHC : 1:500-1:2000
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

82485-1-RR targets KIF5B in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications WB, IHC, IF/ICC, ELISA Application Description
Tested Reactivity human, mouse, rat
Immunogen KIF5B fusion protein Ag16250 种属同源性预测
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Full Name kinesin family member 5B
Synonyms KNS1, KNS, KINH, Kinesin-1 heavy chain, KIF 5B
Calculated Molecular Weight 963 aa, 110 kDa
Observed Molecular Weight 110-120 kDa
GenBank Accession NumberBC126279
Gene Symbol KIF5B
Gene ID (NCBI) 3799
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDP33176
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

KIF5B, also known as KINH, KNS1, belongs to the Kinesin-1 family. KIF5B contains three structural domains: a globular N-terminal motor domain, a central alpha-helical rod domain, and a globular C-terminal domain. During the G2 phase of the cell cycle in a BICD2-dependent manner, KIF5B antagonizes the dynein function and drives the separation of nuclei and centrosomes (PMID: 20386726). KIF5B drives the polarization of cytolytic granules and microtubule-organizing centers (MTOCs) toward the immune synapse between effector NK lymphocytes and target cells (KIF5B:24088571).

实验方案

Product Specific Protocols
WB protocol for KIF5B antibody 82485-1-RRDownload protocol
IHC protocol for KIF5B antibody 82485-1-RRDownload protocol
IF protocol for KIF5B antibody 82485-1-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols