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  • Featured Product
  • KD/KO Validated

CoraLite® Plus 488-conjugated KIF5B Recombinant antibody

KIF5B Uni-rAbTM Recombinant Antibody for IF/ICC, FC (Intra)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

IF/ICC, FC (Intra)

Conjugate

CoraLite® Plus 488 Fluorescent Dye

CloneNo.

2B18

Cat no : CL488-82485

Print datasheet

Synonyms

KNS1, KNS, KINH, Kinesin-1 heavy chain, KIF 5B

验证数据展示

  • IF Staining of HepG2 using CL488-82485

    IF Staining of HepG2 using CL488-82485

    Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using CoraLite® Plus 488 KIF5B antibody (CL488-82485, Clone: 2B18 ) at dilution of 1:200.

  • FC experiment of HepG2 using CL488-82485

    FC experiment of HepG2 using CL488-82485

    1x10^6 HepG2 cells were intracellularly stained with 0.4 ug CoraLite® Plus 488-conjugated KIF5B Recombinant antibody (CL488-82485, Clone:2B18)(red), or 0.4 ug CoraLite® Plus 488-conjugated Rabbit IgG control Rabbit PolyAb (CL488-30000) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

经过测试的应用

Positive IF/ICC detected inHepG2 cells
Positive FC (Intra) detected inHepG2 cells
Planning an IHC experiment? We recommend our IHCeasy KIF5B Ready-To-Use IHC Kit. KIF5B primary antibody included.
For other applications, we recommend the unconjugated version of this antibody, 82485-1-PBS

推荐稀释比

ApplicationDilution
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL488-82485 targets KIF5B in IF/ICC, FC (Intra) applications and shows reactivity with human, mouse, rat samples.

Tested Applications IF/ICC, FC (Intra) Application Description
Tested Reactivity human, mouse, rat
Immunogen KIF5B fusion protein Ag16250 种属同源性预测
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Full Name kinesin family member 5B
Synonyms KNS1, KNS, KINH, Kinesin-1 heavy chain, KIF 5B
Calculated Molecular Weight 963 aa, 110 kDa
Observed Molecular Weight 110-120 kDa
GenBank Accession NumberBC126279
Gene Symbol KIF5B
Gene ID (NCBI) 3799
Conjugate CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission Maxima Wavelengths493 nm / 522 nm
Form Liquid
Purification MethodProtein A purification
UNIPROT IDP33176
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

KIF5B, also known as KINH, KNS1, belongs to the Kinesin-1 family. KIF5B contains three structural domains: a globular N-terminal motor domain, a central alpha-helical rod domain, and a globular C-terminal domain. During the G2 phase of the cell cycle in a BICD2-dependent manner, KIF5B antagonizes the dynein function and drives the separation of nuclei and centrosomes (PMID: 20386726). KIF5B drives the polarization of cytolytic granules and microtubule-organizing centers (MTOCs) toward the immune synapse between effector NK lymphocytes and target cells (KIF5B:24088571).

实验方案

Product Specific Protocols
IF protocol for CL Plus 488 KIF5B antibody CL488-82485Download protocol
FC protocol for CL Plus 488 KIF5B antibody CL488-82485Download protocol
Standard Protocols
Click here to view our Standard Protocols