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验证数据展示

  • IF Staining of HepG2 using CL488-21632

    IF Staining of HepG2 using CL488-21632

    Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using CoraLite® Plus 488 KIF5B antibody (CL488-21632) at dilution of 1:200.

  • Featured Product
  • KD/KO验证

CoraLite® Plus 488-conjugated KIF5B Polyclonal antibody

KIF5B Polyclonal Antibody for IF/ICC
Cat No. CL488-21632

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

IF/ICC

别名

KIF5B, Kinesin 1 heavy chain, kinesin family member 5B, KINH, KNS, KNS1, Ubiquitous kinesin heavy chain, UKHC

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  CoraLite® Plus 488
规格: 

-/ -

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经过测试的应用

Positive IF/ICC detected inHepG2 cells
Planning an IHC experiment? We recommend our IHCeasy KIF5B Ready-To-Use IHC Kit. KIF5B primary antibody included.
For other applications, we recommend the unconjugated version of this antibody, 21632-1-AP

推荐稀释比

应用推荐稀释比
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL488-21632 targets KIF5B in IF/ICC applications and shows reactivity with human, mouse, rat samples.

经测试应用 IF/ICC Application Description
经测试反应性 human, mouse, rat
免疫原 KIF5B fusion protein Ag16250 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 kinesin family member 5B
别名 KIF5B, Kinesin 1 heavy chain, kinesin family member 5B, KINH, KNS, KNS1, Ubiquitous kinesin heavy chain, UKHC
计算分子量 963 aa, 110 kDa
观测分子量 110-120 kDa
GenBank蛋白编号BC126279
基因名称 KIF5B
Gene ID (NCBI) 3799
RRIDAB_3084054
偶联类型 CoraLite® Plus 488 Fluorescent Dye
最大激发/发射波长493 nm / 522 nm
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDP33176
储存缓冲液 PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA , pH 7.3
储存条件Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

KIF5B, also known as KINH, KNS1, belongs to the Kinesin-1 family. KIF5B contains three structural domains: a globular N-terminal motor domain, a central alpha-helical rod domain, and a globular C-terminal domain. During the G2 phase of the cell cycle in a BICD2-dependent manner, KIF5B antagonizes the dynein function and drives the separation of nuclei and centrosomes (PMID: 20386726). KIF5B drives the polarization of cytolytic granules and microtubule-organizing centers (MTOCs) toward the immune synapse between effector NK lymphocytes and target cells (KIF5B:24088571).

实验方案

Product Specific Protocols
IF protocol for CL Plus 488 KIF5B antibody CL488-21632Download protocol
Standard Protocols
Click here to view our Standard Protocols