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验证数据展示

  • WB analysis of HeLa using 12313-1-AP

    WB analysis of HeLa using 12313-1-AP

    HeLa cells, mouse testis tissue, and rat testis tissue were subjected to SDS PAGE followed by western blot with 12313-1-AP (KIN17 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • IP experiment of mouse brain using 12313-1-AP

    IP experiment of mouse brain using 12313-1-AP

    IP result of anti-KIN17 (IP:12313-1-AP, 4ug; Detection:12313-1-AP 1:500) with mouse brain tissue lysate 3160 ug.

  • IF Staining of HeLa using 12313-1-AP

    IF Staining of HeLa using 12313-1-AP

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using KIN17 antibody (12313-1-AP) at dilution of 1:600 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002), CL594-phalloidin (red).

  • IHC staining of human ovary cancer using 12313-1-AP

    IHC staining of human ovary cancer using 12313-1-AP

    Immunohistochemical analysis of paraffin-embedded human ovary cancer tissue slide using 12313-1-AP (KIN17 antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with KIN antibody included

KIN17 Polyclonal antibody

KIN17 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA
Cat No. 12313-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse

应用

WB, IHC, IF/ICC, IP, ELISA

别名

KIN, DNA/RNA-binding protein KIN17, DNA/RNA binding protein KIN17, BTCD, Binding to curved DNA

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
CoraLite® Plus 488
规格: 

-/ -

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经过测试的应用

Positive WB detected inHeLa cells, human heart tissue
Positive IP detected inmouse brain tissue
Positive IHC detected inhuman ovary cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHeLa cells
Planning an IHC experiment? We recommend our IHCeasy KIN Ready-To-Use IHC Kit. KIN primary antibody included.
Planning an IF experiment? We recommend our CoraLite® Plus 488 conjugated versions of this antibody.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:1000-1:4000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:300-1:1200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

12313-1-AP targets KIN17 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse samples.

经测试应用 WB, IHC, IF/ICC, IP, ELISA Application Description
经测试反应性 human, mouse
免疫原 KIN17 fusion protein Ag2963 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 KIN, antigenic determinant of recA protein homolog (mouse)
别名 KIN, DNA/RNA-binding protein KIN17, DNA/RNA binding protein KIN17, BTCD, Binding to curved DNA
计算分子量 393 aa, 45 kDa
观测分子量 40 kDa
GenBank蛋白编号BC017309
基因名称 KIN17
Gene ID (NCBI) 22944
RRIDAB_2130343
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDO60870
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

KIN17 (also known as KIN) is an evolutionarily conserved zinc finger nuclear protein. It consists of three motifs, a zinc finger, a nuclear localization signal and a Kyprides-Onzonis-Woese motif that mediates transcription elongation. KIN17 is ubiquitously expressed in human tissues and participates in DNA replication, DNA repair and cell cycle progression. Emerging evidence has indicated the importance of KIN17 in the pathogenesis and progression of tumors (PMID: 28454391). KIN17 has a calculated molecular wight of 45 kDa, this antibody is raised against human KIN17 and detects a band of about 40 kDa.

实验方案

Product Specific Protocols
WB protocol for KIN17 antibody 12313-1-APDownload protocol
IHC protocol for KIN17 antibody 12313-1-APDownload protocol
IF protocol for KIN17 antibody 12313-1-APDownload protocol
IP protocol for KIN17 antibody 12313-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols