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KIN17 Polyclonal antibody

KIN17 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, IF/ICC, IP, ELISA

Conjugate

Unconjugated

Cat no : 12313-1-AP

Print datasheet

Synonyms

KIN, DNA/RNA-binding protein KIN17, DNA/RNA binding protein KIN17, BTCD, Binding to curved DNA

验证数据展示

  • WB analysis of HeLa using 12313-1-AP

    WB analysis of HeLa using 12313-1-AP

    HeLa cells, mouse testis tissue, and rat testis tissue were subjected to SDS PAGE followed by western blot with 12313-1-AP (KIN17 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • IP experiment of mouse brain using 12313-1-AP

    IP experiment of mouse brain using 12313-1-AP

    IP result of anti-KIN17 (IP:12313-1-AP, 4ug; Detection:12313-1-AP 1:500) with mouse brain tissue lysate 3160 ug.

  • IF Staining of HeLa using 12313-1-AP

    IF Staining of HeLa using 12313-1-AP

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using KIN17 antibody (12313-1-AP) at dilution of 1:600 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002), CL594-phalloidin (red).

  • IHC staining of human ovary cancer using 12313-1-AP

    IHC staining of human ovary cancer using 12313-1-AP

    Immunohistochemical analysis of paraffin-embedded human ovary cancer tissue slide using 12313-1-AP (KIN17 antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with KIN antibody included

经过测试的应用

Positive WB detected inHeLa cells, human heart tissue
Positive IP detected inmouse brain tissue
Positive IHC detected inhuman ovary cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHeLa cells
Planning an IHC experiment? We recommend our IHCeasy KIN Ready-To-Use IHC Kit. KIN primary antibody included.
Planning an IF experiment? We recommend our CoraLite® Plus 488 conjugated versions of this antibody.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:4000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:300-1:1200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

12313-1-AP targets KIN17 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse samples.

Tested Applications WB, IHC, IF/ICC, IP, ELISA Application Description
Tested Reactivity human, mouse
Immunogen KIN17 fusion protein Ag2963 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name KIN, antigenic determinant of recA protein homolog (mouse)
Synonyms KIN, DNA/RNA-binding protein KIN17, DNA/RNA binding protein KIN17, BTCD, Binding to curved DNA
Calculated Molecular Weight 393 aa, 45 kDa
Observed Molecular Weight 40 kDa
GenBank Accession NumberBC017309
Gene Symbol KIN17
Gene ID (NCBI) 22944
RRIDAB_2130343
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDO60870
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

KIN17 (also known as KIN) is an evolutionarily conserved zinc finger nuclear protein. It consists of three motifs, a zinc finger, a nuclear localization signal and a Kyprides-Onzonis-Woese motif that mediates transcription elongation. KIN17 is ubiquitously expressed in human tissues and participates in DNA replication, DNA repair and cell cycle progression. Emerging evidence has indicated the importance of KIN17 in the pathogenesis and progression of tumors (PMID: 28454391). KIN17 has a calculated molecular wight of 45 kDa, this antibody is raised against human KIN17 and detects a band of about 40 kDa.

实验方案

Product Specific Protocols
WB protocol for KIN17 antibody 12313-1-APDownload protocol
IHC protocol for KIN17 antibody 12313-1-APDownload protocol
IF protocol for KIN17 antibody 12313-1-APDownload protocol
IP protocol for KIN17 antibody 12313-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols