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CoraLite® Plus 488-conjugated KIN17 Polyclonal antibody

KIN17 Polyclonal Antibody for IF/ICC, FC (Intra)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

IF/ICC, FC (Intra)

Conjugate

CoraLite® Plus 488 Fluorescent Dye

Cat no : CL488-12313

Print datasheet

Synonyms

KIN, DNA/RNA-binding protein KIN17, DNA/RNA binding protein KIN17, BTCD, Binding to curved DNA

验证数据展示

  • IF Staining of HeLa using CL488-12313

    IF Staining of HeLa using CL488-12313

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using CoraLite® Plus 488 KIN17 antibody (CL488-12313) at dilution of 1:200.

  • FC experiment of HeLa using CL488-12313

    FC experiment of HeLa using CL488-12313

    1x10^6 HeLa cells were intracellularly stained with 0.8 ug Coralite® Plus 488 KIN17 Polyclonal Antibody (CL488-12313) (red), or 0.8 ug CoraLite® Plus 488-conjugated Rabbit IgG control Rabbit PolyAb (CL488-30000) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

经过测试的应用

Positive IF/ICC detected inHeLa cells
Positive FC (Intra) detected inHeLa cells
Planning an IHC experiment? We recommend our IHCeasy KIN Ready-To-Use IHC Kit. KIN primary antibody included.
For other applications, we recommend the unconjugated version of this antibody, 12313-1-AP

推荐稀释比

ApplicationDilution
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.80 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL488-12313 targets KIN17 in IF/ICC, FC (Intra) applications and shows reactivity with human, mouse, rat samples.

Tested Applications IF/ICC, FC (Intra) Application Description
Tested Reactivity human, mouse, rat
Immunogen KIN17 fusion protein Ag2963 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name KIN, antigenic determinant of recA protein homolog (mouse)
Synonyms KIN, DNA/RNA-binding protein KIN17, DNA/RNA binding protein KIN17, BTCD, Binding to curved DNA
Calculated Molecular Weight 393 aa, 45 kDa
Observed Molecular Weight 40 kDa
GenBank Accession NumberBC017309
Gene Symbol KIN17
Gene ID (NCBI) 22944
Conjugate CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission Maxima Wavelengths493 nm / 522 nm
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDO60870
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

KIN17 (also known as KIN) is an evolutionarily conserved zinc finger nuclear protein. It consists of three motifs, a zinc finger, a nuclear localization signal and a Kyprides-Onzonis-Woese motif that mediates transcription elongation. KIN17 is ubiquitously expressed in human tissues and participates in DNA replication, DNA repair and cell cycle progression. Emerging evidence has indicated the importance of KIN17 in the pathogenesis and progression of tumors (PMID: 28454391). KIN17 has a calculated molecular wight of 45 kDa, this antibody is raised against human KIN17 and detects a band of about 40 kDa.

实验方案

Product Specific Protocols
IF protocol for CL Plus 488 KIN17 antibody CL488-12313Download protocol
FC protocol for CL Plus 488 KIN17 antibody CL488-12313Download protocol
Standard Protocols
Click here to view our Standard Protocols