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验证数据展示

  • IF Staining of HeLa using CL488-12313

    IF Staining of HeLa using CL488-12313

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using CoraLite® Plus 488 KIN17 antibody (CL488-12313) at dilution of 1:200.

  • FC experiment of HeLa using CL488-12313

    FC experiment of HeLa using CL488-12313

    1x10^6 HeLa cells were intracellularly stained with 0.8 ug Coralite® Plus 488 KIN17 Polyclonal Antibody (CL488-12313) (red), or 0.8 ug CoraLite® Plus 488-conjugated Rabbit IgG control Rabbit PolyAb (CL488-30000) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

CoraLite® Plus 488-conjugated KIN17 Polyclonal antibody

KIN17 Polyclonal Antibody for IF/ICC, FC (Intra)
Cat No. CL488-12313

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

IF/ICC, FC (Intra)

别名

KIN, DNA/RNA-binding protein KIN17, DNA/RNA binding protein KIN17, BTCD, Binding to curved DNA

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  CoraLite® Plus 488
规格: 

-/ -

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经过测试的应用

Positive IF/ICC detected inHeLa cells
Positive FC (Intra) detected inHeLa cells
Planning an IHC experiment? We recommend our IHCeasy KIN Ready-To-Use IHC Kit. KIN primary antibody included.
For other applications, we recommend the unconjugated version of this antibody, 12313-1-AP

推荐稀释比

应用推荐稀释比
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.80 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL488-12313 targets KIN17 in IF/ICC, FC (Intra) applications and shows reactivity with human, mouse, rat samples.

经测试应用 IF/ICC, FC (Intra) Application Description
经测试反应性 human, mouse, rat
免疫原 KIN17 fusion protein Ag2963 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 KIN, antigenic determinant of recA protein homolog (mouse)
别名 KIN, DNA/RNA-binding protein KIN17, DNA/RNA binding protein KIN17, BTCD, Binding to curved DNA
计算分子量 393 aa, 45 kDa
观测分子量 40 kDa
GenBank蛋白编号BC017309
基因名称 KIN17
Gene ID (NCBI) 22944
RRIDAB_3672535
偶联类型 CoraLite® Plus 488 Fluorescent Dye
最大激发/发射波长493 nm / 522 nm
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDO60870
储存缓冲液 PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA , pH 7.3
储存条件Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

KIN17 (also known as KIN) is an evolutionarily conserved zinc finger nuclear protein. It consists of three motifs, a zinc finger, a nuclear localization signal and a Kyprides-Onzonis-Woese motif that mediates transcription elongation. KIN17 is ubiquitously expressed in human tissues and participates in DNA replication, DNA repair and cell cycle progression. Emerging evidence has indicated the importance of KIN17 in the pathogenesis and progression of tumors (PMID: 28454391). KIN17 has a calculated molecular wight of 45 kDa, this antibody is raised against human KIN17 and detects a band of about 40 kDa.

实验方案

Product Specific Protocols
IF protocol for CL Plus 488 KIN17 antibody CL488-12313Download protocol
FC protocol for CL Plus 488 KIN17 antibody CL488-12313Download protocol
Standard Protocols
Click here to view our Standard Protocols