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  • Featured Product
  • KD/KO Validated

KLF5 Polyclonal antibody, PBS Only

KLF5 Polyclonal Antibody for WB, IHC, IF/ICC, IP, Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, IP, Indirect ELISA

Conjugate

Unconjugated

Cat no : 21017-1-PBS

Print datasheet

Synonyms

验证数据展示

  • IP experiment of HeLa using 21017-1-AP (same clone as 21017-1-PBS)

    IP experiment of HeLa using 21017-1-AP (same clone as 21017-1-PBS)

    IP result of anti-KLF5 (IP:21017-1-AP, 4ug; Detection:21017-1-AP 1:500) with HeLa cells lysate 2800ug. This data was developed using the same antibody clone with 21017-1-PBS in a different storage buffer formulation.

  • WB analysis using 21017-1-AP (same clone as 21017-1-PBS)

    WB analysis using 21017-1-AP (same clone as 21017-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 21017-1-AP (KLF5 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 21017-1-PBS in a different storage buffer formulation.

  • WB analysis using 21017-1-AP (same clone as 21017-1-PBS)

    WB analysis using 21017-1-AP (same clone as 21017-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 21017-1-AP (KLF5 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 21017-1-PBS in a different storage buffer formulation.

  • IHC staining of human bowen disease using 21017-1-AP (same clone as 21017-1-PBS)

    IHC staining of human bowen disease using 21017-1-AP (same clone as 21017-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human bowen disease slide using 21017-1-AP (KLF5 antibody) at dilution of 1:4000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 21017-1-PBS in a different storage buffer formulation.

  • IHC staining of human bowen disease using 21017-1-AP (same clone as 21017-1-PBS)

    IHC staining of human bowen disease using 21017-1-AP (same clone as 21017-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human bowen disease slide using 21017-1-AP (KLF5 antibody) at dilution of 1:4000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 21017-1-PBS in a different storage buffer formulation.

  • IHC staining of human colon cancer using 21017-1-AP (same clone as 21017-1-PBS)

    IHC staining of human colon cancer using 21017-1-AP (same clone as 21017-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 21017-1-AP (KLF5 antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 21017-1-PBS in a different storage buffer formulation.

  • IHC staining of human colon cancer using 21017-1-AP (same clone as 21017-1-PBS)

    IHC staining of human colon cancer using 21017-1-AP (same clone as 21017-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 21017-1-AP (KLF5 antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 21017-1-PBS in a different storage buffer formulation.

  • IF Staining of HeLa using 21017-1-AP (same clone as 21017-1-PBS)

    IF Staining of HeLa using 21017-1-AP (same clone as 21017-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using KLF5 antibody (21017-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-phalloidin (red). This data was developed using the same antibody clone with 21017-1-PBS in a different storage buffer formulation.

产品信息

21017-1-PBS targets KLF5 in WB, IHC, IF/ICC, IP, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications WB, IHC, IF/ICC, IP, Indirect ELISA Application Description
Tested Reactivity human, mouse, rat
Immunogen KLF5 fusion protein Ag15267 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name Kruppel-like factor 5 (intestinal)
Synonyms
Calculated Molecular Weight 457 aa, 51 kDa
Observed Molecular Weight 51-55 kDa
GenBank Accession NumberBC042131
Gene Symbol KLF5
Gene ID (NCBI) 688
RRIDAB_10696447
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ13887
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

KLF5, also known as BTEB2 and IKLF, is a member of the Krueppel-like transcription factor family. It is a transcription factor for SMemb/non-muscle myosin heavy chain-B (NMHC-B). It is a molecular marker of phenotypically modulated smooth-muscle cells. KLF5 seems to be a key element linking external stress and cardiovascular remodeling. This antibody detects the 50-60 kDa isoform 1 of KLF5. The antibody can recognize isoform 2 (33 kDa) and isoform 3 (18 kDa) too. 85 kDa, 95 kDa and 120 kDa of SUMOylated KLF5 were detected (PMID: 18500350).