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KLF5 Recombinant antibody

KLF5 Recombinant Antibody for IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

Human

Applications

IF/ICC, ELISA

Conjugate

Unconjugated

CloneNo.

230509G7

Cat No : 83205-4-RR

Print datasheet

Synonyms

BTE binding protein 2, BTEB2, CKLF, Colon krueppel like factor, GC box binding protein 2, IKLF, KLF5, Krueppel like factor 5, Transcription factor BTEB2

验证数据展示

  • IF Staining of HeLa using 83205-4-RR

    IF Staining of HeLa using 83205-4-RR

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using KLF5 antibody (83205-4-RR, Clone: 230509G7 ) at dilution of 1:800 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).

  • Affinity experiments of 83205-4-RR

    Affinity experiments of 83205-4-RR

    Biolayer interferometry (BLl) kinetic assays of 83205-4-RR against Human KLF5 were performed. The affinity constant is 2.36 nM.

经过测试的应用

Positive IF detected inHeLa cells

推荐稀释比

ApplicationDilution
Immunofluorescence (IF)IF : 1:400-1:1600
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

The immunogen of 83205-4-RR is KLF5 Fusion Protein expressed in E. coli.

Tested Applications IF/ICC, ELISA Application Description
Tested Reactivity Human
Immunogen KLF5 fusion protein Ag15267 种属同源性预测
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Full Name Kruppel-like factor 5 (intestinal)
Synonyms BTE binding protein 2, BTEB2, CKLF, Colon krueppel like factor, GC box binding protein 2, IKLF, KLF5, Krueppel like factor 5, Transcription factor BTEB2
Calculated Molecular Weight 457 aa, 51 kDa
Observed Molecular Weight 51-57 kDa
GenBank Accession NumberBC042131
Gene Symbol KLF5
Gene ID (NCBI) 688
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
UNIPROT IDQ13887
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

KLF5, also known as BTEB2 and IKLF, is a member of the Krueppel-like transcription factor family. It is a transcription factor for SMemb/non-muscle myosin heavy chain-B (NMHC-B). It is a molecular marker of phenotypically modulated smooth-muscle cells. KLF5 seems to be a key element linking external stress and cardiovascular remodeling.

实验方案

Product Specific Protocols
IF protocol for KLF5 antibody 83205-4-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols