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CoraLite® Plus 488-conjugated KLK3/PSA Recombinant antibody

KLK3/PSA Uni-rAbTM Recombinant Antibody for IF/ICC, FC (Intra)

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

IF/ICC, FC (Intra)

Conjugate

CoraLite® Plus 488 Fluorescent Dye

CloneNo.

241263F8

Cat no : CL488-84059-5

Print datasheet

Synonyms

KLK3, Kallikrein-3, Kallikrein 3, Gamma-seminoprotein, EC:3.4.21.77

验证数据展示

  • IF Staining of LNCaP using CL488-84059-5

    IF Staining of LNCaP using CL488-84059-5

    Immunofluorescent analysis of (4% PFA) fixed LNCaP cells using CoraLite® Plus 488 KLK3/PSA antibody (CL488-84059-5, Clone: 241263F8 ) at dilution of 1:200.

  • FC experiment of PC-3 using CL488-84059-5

    FC experiment of PC-3 using CL488-84059-5

    1x10^6 PC-3 cells were intracellularly stained with 0.25 ug CoraLite® Plus 488-conjugated KLK3/PSA Recombinant antibody (CL488-84059-5, Clone:241263F8)(red), or 0.25 ug CoraLite® Plus 488 Rabbit IgG Isotype Control Recombinant Antibody (CL488-98136, Clone: 240953C9) (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

  • FC experiment of HeLa using CL488-84059-5

    FC experiment of HeLa using CL488-84059-5

    1x10^6 HeLa cells were intracellularly stained with 0.25 ug CoraLite® Plus 488-conjugated KLK3/PSA Recombinant antibody (CL488-84059-5, Clone:241263F8)(red), or 0.25 ug CoraLite® Plus 488 Rabbit IgG Isotype Control Recombinant Antibody (CL488-98136, Clone: 240953C9) (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

经过测试的应用

Positive IF/ICC detected inLNCaP cells
Positive FC (Intra) detected inPC-3 cells, HeLa cells
Planning an IHC experiment? We recommend our IHCeasy PSA Ready-To-Use IHC Kit. PSA primary antibody included.
For other applications, we recommend the unconjugated version of this antibody, 84059-5-PBS

推荐稀释比

ApplicationDilution
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL488-84059-5 targets KLK3/PSA in IF/ICC, FC (Intra) applications and shows reactivity with human samples.

Tested Applications IF/ICC, FC (Intra) Application Description
Tested Reactivity human
Immunogen Fusion Protein 种属同源性预测
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Full Name kallikrein-related peptidase 3
Synonyms KLK3, Kallikrein-3, Kallikrein 3, Gamma-seminoprotein, EC:3.4.21.77
Calculated Molecular Weight29 kDa
Observed Molecular Weight 30-34 kDa
GenBank Accession NumberNM_001648.2
Gene Symbol KLK3
Gene ID (NCBI) 354
Conjugate CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission Maxima Wavelengths493 nm / 522 nm
Form Liquid
Purification MethodProtein A purification
UNIPROT IDP07288-1
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Kallikreins are a subgroup of serine proteases having diverse physiological functions. Growing evidence suggests that many kallikreins are implicated in carcinogenesis and some have potential as novel cancer and other disease biomarkers. kallikrein-related peptidase 3, which is also called PSA (prostate-specific antigen) in the clinical setting, is useful in the diagnosis and monitoring of prostatic carcinoma. KLK3 has 5 isoforms  produced by alternative splicing.

实验方案

Product Specific Protocols
IF protocol for CL Plus 488 KLK3/PSA antibody CL488-84059-5Download protocol
Standard Protocols
Click here to view our Standard Protocols