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KLK7 Polyclonal antibody

KLK7 Polyclonal Antibody for WB, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IF/ICC, ELISA

Conjugate

Unconjugated

Cat no : 23810-1-AP

Print datasheet

Synonyms

SCCE, PRSS6, Kallikrein-7, EC:3.4.21.117

验证数据展示

  • WB analysis using 23810-1-AP

    WB analysis using 23810-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 23810-1-AP (KLK7 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

  • IF Staining of A431 using 23810-1-AP

    IF Staining of A431 using 23810-1-AP

    Immunofluorescent analysis of (-20°C Methanol) fixed A431 cells using KLK7 antibody (23810-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

经过测试的应用

Positive WB detected inmouse skin tissue, rat skin tissue
Positive IF/ICC detected inA431 cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

23810-1-AP targets KLK7 in WB, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications WB, IF/ICC, ELISA Application Description
Tested Reactivity human, mouse, rat
Immunogen KLK7 fusion protein Ag20760 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name kallikrein-related peptidase 7
Synonyms SCCE, PRSS6, Kallikrein-7, EC:3.4.21.117
Calculated Molecular Weight 28 kDa
Observed Molecular Weight 27-30 kDa
GenBank Accession NumberBC032005
Gene Symbol KLK7
Gene ID (NCBI) 5650
RRIDAB_3669417
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen Affinity purified
UNIPROT IDP49862
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Kallikrein-7(KLK7), also known as SCCE, PRSS6, is a member of the kallikrein subfamily of serine proteases involved in various enzymatic processes. KLK7 is abundantly expressed in the skin and is expressed by keratinocytes in the epidermis(PMID: 10974542).

实验方案

Product Specific Protocols
WB protocol for KLK7 antibody 23810-1-APDownload protocol
IF protocol for KLK7 antibody 23810-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols